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pAAV-U6-DR36(DjCas13d-SapI)-CMV-intron-MCS-pA

Plasmid

#233031

Purpose

To express a DjCas13d-compatible gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d-compatible gRNA expression cassette

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-P2A-mCherry-pA

Plasmid

#233027

Purpose

To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-P2A-mCherry-pA

Plasmid

#233025

Purpose

To Express HA tagged CasRX and mcherry from the mammalian EFS promoter. The CasRx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-P2A-mCherry-pA

Plasmid

#233026

Purpose

To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SOX17

Plasmid

#222925

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOX17 (SOX17 Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

Plasmid

#208873

Purpose

For expression in E. coli of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

Plasmid

#208863

Purpose

Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

Plasmid

#208864

Purpose

Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

I11S/L12S (disrupts FIP200 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208865

Purpose

Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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CSII-PPM1H-mApple-mito

Plasmid

#207351

Purpose

Mammalian expression of PPm1H-mito

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPm1H-mito (PPM1H Human)

Expression

Mammalian

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S124A

Plasmid

#208368

Purpose

Mammalian expression of HA-tagged human PPM1H S124A

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S124A (PPM1H Human)

Expression

Mammalian

Mutation

S124A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S211A

Plasmid

#208369

Purpose

Mammalian expression of HA-tagged human PPM1H S211A

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S211A (PPM1H Human)

Expression

Mammalian

Mutation

S211A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ10 PPM1H

Plasmid

#207335

Purpose

Mammalian expression of HA-Δ10 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ10

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ20 PPM1H

Plasmid

#207336

Purpose

Mammalian expression of HA-Δ20 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ20

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ34 PPM1H

Plasmid

#207337

Purpose

Mammalian expression of HA-Δ34 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ34

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37 PPM1H

Plasmid

#207338

Purpose

Mammalian expression of HA-Δ37 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ44 PPM1H

Plasmid

#207339

Purpose

Mammalian expression of HA-Δ44 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ44

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ71 PPM1H

Plasmid

#207340

Purpose

Mammalian expression of HA-Δ71 PPM1H

Depositor

Suzanne Pfeffer

Article

Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ71

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PRPF4-1

Plasmid

#156158

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF4 (PRPF4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB96

Plasmid

#199316

Purpose

MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR

Expression

Worm

Mutation

mTagBFP2 from pJJR81, C. elegans codon optimized …

Promoter

flp-18

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBVboostFGII WPRE 3C-CVB3

Plasmid

#202077

Purpose

Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB3 3C protease with HisTag

Tags

6xHis-tag

Expression

Bacterial, Insect, and Mamm…

Promoter

polh, CAG, T7

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Δ1-198)

Plasmid

#186222

Purpose

For expression of ATG13(Δ1-198) tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deleted amino acids 1 - 198

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

Plasmid

#198037

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

E696K

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-HsNot1_1093-1317_AF

Plasmid

#148699

Purpose

Bacterial Expression of HsNot1_1093-1317

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1_1093-1317 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1093-2376_AE

Plasmid

#148629

Purpose

Bacterial Expression of HsNot1iso1_1093-2376

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1iso1_1093-2376 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot1iso1_1607-1815_AE

Plasmid

#148631

Purpose

Bacterial Expression of HsNot1iso1_1607-1815

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsNot1iso1_1607-1815 (CNOT1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-DmRoquin_702-819-del725-755-del790-812_AE

Plasmid

#148606

Purpose

Insect Expression of DmRoquin_702-819-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755-del790-812 (roq Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del725-755-del790-812-GB1His6_AE

Plasmid

#148621

Purpose

Bacterial Expression of DmRoquin_702-819-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755-del790-812 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1497-1644_W

Plasmid

#147872

Purpose

Insect Expression of DmNot1_1497-1644

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1497-1644 (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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