We narrowed to 8,227 results for: sars

1. B-HeFSpCas9-A1003K

   Plasmid

   #207371

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1003K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

   Promoter

   CBh

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-HeFSpCas9-A848K

   Plasmid

   #207369

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A848K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-HeFSpCas9-A661R

   Plasmid

   #207367

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-evoSpCas9-Q661R

   Plasmid

   #207366

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-Q661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-evoSpCas9-N515Y

   Plasmid

   #207364

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-N515Y
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. HypaR-SpCas9

   Plasmid

   #126757

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-HypaSpCas9

   Plasmid

   #126763

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A; M694A; Q695A; H698A; amino acids 1005-1013…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid

    #126755

    Purpose

    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MLRV

    Plasmid

    #118069

    Purpose

    Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Inserts

    TB:5xNF-κβ:RedF:bGHpA
    TB:4xTGF-β:FLuc:bGHpA
    TB:5xE-box:Renilla:bGHpA
    TB:2xp53:NLuc:bGHpA
    TB:6xAP-1:GrRenilla:bGHpA
    CMV:ELuc:bGHpA
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNK7187

    Plasmid

    #219761

    Purpose

    MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1-FLAG-hSF3B1-K700E

    Plasmid

    #82577

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-K700E (SF3B1 Human)
    

    Use

    Tags

    FLAG peptide

    Expression

    Mammalian

    Mutation

    K700E

    Promoter

    CMV

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDF-Mm2

    Plasmid

    #197100

    Purpose

    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    PylRS variant, Pyl tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcBac1-Mb-sfGFP WT

    Plasmid

    #197569

    Purpose

    Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22-His-TEV-DOT1L

    Plasmid

    #89610

    Purpose

    Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

    Depositor

    Leemor Joshua-Tor

    Article

    Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

    Insert

    Dot1L (DOT1L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpCas9-HF1-plus

    Plasmid

    #126768

    Purpose

    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. scAAV-TTR-mFgf15

    Plasmid

    #190594

    Purpose

    AAV construct containing Fgf15 cds under control of TTR promoter

    Depositor

    Roel Nusse

    Article

    Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

    Insert

    Fgf15 (Fgf15 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ttr

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNK7190

    Plasmid

    #219763

    Purpose

    MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    nnLuz, nnH3H, nnCPH
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNK7183

    Plasmid

    #219760

    Purpose

    MoClo-compatible Level 1 for expression PzPKS2 in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PzPKS2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNK7180

    Plasmid

    #219762

    Purpose

    MoClo-compatible Level 1 for expression HmS in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    HmS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Creb5 shRNA

    Plasmid

    #195020

    Purpose

    Bovine Creb5 shRNA targeting the Creb5 3′UTR

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Creb5 shRNA (CREB5 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GAL4-Creb5 (1- 128) T59/T61 A

    Plasmid

    #195032

    Purpose

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with T59/T61 A mutation)

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with with T59/T61 A mutation) (CREB5 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T59/T61 A

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GAL4-Creb5 (1- 128) C18/C23 S

    Plasmid

    #195033

    Purpose

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation)

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa, with C18/C23 S mutation) (CREB5 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C18/C23 S

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. B-HypaR-SpCas9

    Plasmid

    #126764

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A; N692A; M694A; Q695A; H698A; amino acids 10…

    Promoter

    CBh

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS-CD4-YEML-myc

    Plasmid

    #58538

    Purpose

    Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…

    Expression

    Mammalian

    Mutation

    Added C-terminal Tyrosine-Glutamate-Methionine-Le…

    Promoter

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

28. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid

    #92120

    Purpose

    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDH-EF1a-hSNCA

    Plasmid

    #170445

    Purpose

    Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human SNCA (SNCA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAcBac1-Mb-sfGFP 150TAG

    Plasmid

    #197570

    Purpose

    Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge