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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,603 results for: sars

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Showing: 4981 - 5010 of 8603 results

  15. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92116-plasmid-map-sequence-id-177904

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  18. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92119-plasmid-map-sequence-id-194893

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  22. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

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  23. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

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  24. K15/pGL3

    Plasmid
    #44264
    Depositor
    George Cotsarelis
    Article
    Liu et al J Invest Dermatol. 2003 Nov;121(5):963-8
    Insert
    K15 promoter (-4.8) (Krt15 Mouse)
    Use
    Luciferase and Mouse Targeting
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Contains the murine K15 promoter (-4.8) fragment
    Promoter
    murine K15 promoter (-4.8) fragment
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. M7138

    Plasmid
    #219774
    Purpose
    MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of ORCA3 promoter and terminator
    Depositor
    Karen Sarkisyan
    Article
    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://
    Insert
    pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219774-plasmid-map-sequence-id-437302

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  26. M7691

    Plasmid
    #219775
    Purpose
    MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of WRKY70 promoter and terminator
    Depositor
    Karen Sarkisyan
    Article
    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://
    Insert
    pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219775-plasmid-map-sequence-id-437303

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  27. pNK3558

    Plasmid
    #219776
    Purpose
    MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of p35S promoter and act2 terminator
    Depositor
    Karen Sarkisyan
    Article
    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://
    Insert
    pNOS-HygR-ocsT | p35S-nnLuz-act2
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    pNOS-HygR-ocsT | p35S-nnLuz-act2
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219776-plasmid-map-sequence-id-438202

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Showing: 4981 - 5010 of 8603 results