We narrowed to 17,418 results for: She
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pUASp YFP Cdc42 CA
Plasmid#52250PurposeDrosophila expression of YFP-Cdc42 constitutively active mutantDepositorInsertCdc42 (Cdc42 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ61L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Nanog(L122A)-IP
Plasmid#62992PurposeExpresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).DepositorInsertMouse Nanog L122A Mutant (Nanog Mouse)
UseTagsExpressionMammalianMutationChanged Leucine 122 (123 AA in Mouse Protein) to …PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
mouse pGL3-ank3_1-promoter firefly luciferase
Plasmid#86436PurposeMouse pGL3-ank3_1-promoter firefly luciferase, ca 2000 base pairs around TSS exon 1eDepositorInsertank3_1 promoter
UseTagsn/aExpressionMammalianMutationn/aPromoterankyrin-3_1Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-G protein gamma-10
Plasmid#67041PurposeG protein gamma-10 tagged with HaloTag on amino terminusDepositorInsertHaloTag-G protein gamma-10 (GNG10 Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKG194
Plasmid#69757PurposeMammalian Expression of CENP-T aa 1-375DepositorInsertCENP-T aa 1-375 (CENPT Human)
UseRetroviralTagsLacI-NLS and eGFPExpressionMammalianMutatione.coli optimized sequencePromoterAvailable sinceNov. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP10
Plasmid#159616PurposeBacterial expression plasmid for COVID-SARS2 NSP10 fused to GSTDepositorInsertCovid-SARS2 Nsp10
UseTagsGST-3C protease siteExpressionBacterialMutationPromoterT7 - LacOAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
RFXANK (3UXG)
Plasmid#36902PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRFXANK (RFXANK Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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FL-DYSF-pUC57
Plasmid#60177PurposePlasmid contains full-length human dysferlin in a multiple cloning site of pUC57-Kan and can be easily cloned into other vectors.DepositorInsertDysferlin (DYSF Human)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PS-CFP2-Alpha-5-Integrin-12
Plasmid#62794PurposeLocalization: Focal Adhesions, Excitation:400 / 490, Emission:468 / 511DepositorInsertAlpha5Integrin (ITGAV Human)
UseTagsCFPExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIC146
Plasmid#63069PurposeRetroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)DepositorInsertCENP-P, IMAGE clone 5554572 (CENPP Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pLV Lnpk-V5
Plasmid#175121PurposeLentiviral expression of mouse Lnpk-V5DepositorInsertLnpk (Lnpk Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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KSP_K406
Plasmid#129771PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKSP (kif11.L Frog)
UseTagsHis6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
taRAJ31min
Plasmid#69934PurposeExpresses taRAJ31min, a linear riboregulator.DepositorInsertplTet-taRAJ31min
UseSynthetic BiologyTagsExpressionMutationPromoterPltetO1-2Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-FOXO WT
Plasmid#52982Purposeinsect expression of FOXO WTDepositorInsertFOXO (foxo Fly)
UseTagsHAExpressionInsectMutationPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)
Plasmid#44087DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)
Plasmid#44078DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
hKv2.1-DN-HA
Plasmid#111530PurposeExpresses a non-conducting dominant neg in mammalian cellsDepositorInserthuman W369C-Y384T-Kv2.1 + HA (KCNB1 Human)
UseTagsHAExpressionMammalianMutationW369C-Y384TPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)
Plasmid#44088DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)
Plasmid#44075DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK276
Plasmid#73366PurposeEncodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)DepositorInsertplastid omega-3 fatty acid desaturase
UseTagsExpressionYeastMutationMutation I231F. Also insert is missing residues 1…PromoterGAL1Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SET7-Hygro
Plasmid#104407Purposetransient expression of dCas9-SET7 fusion proteinDepositorInsertSET7 (SETD7 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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USP37
Plasmid#36898PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP37 (USP37 Human)
UseTagsHis tag with thrombin cleavageExpressionBacterialMutationcontains amino acid residues 4-125 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)
Plasmid#44066DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, His3…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 APBB3 WW domain
Plasmid#104262PurposeBacterial expression of WW domain from APBB3DepositorInsertAPBB3 WW domain (APBB3 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 APBB2 WW domain
Plasmid#104257PurposeBacterial expression of WW domain from APBB2DepositorInsertAPBB2 WW domain (APBB2 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)
Plasmid#44064DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAsylum
Plasmid#24754DepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rholike DN
Plasmid#52240PurposeDrosophila expression of YFP-Rholike dominant negative mutantDepositorInsertRholike (RhoL Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT25N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAc.1B-EGFP-DmHrb98
Plasmid#66595PurposeExpresses EGFP-tagged Hrb98 in Drosophila cellsDepositorInsertHrb98 (Hrb98DE Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterActin 5CAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Lhx2
Plasmid#45873DepositorInsertLhx2 in situ probe (Lhx2 Mouse)
UseIn situTagsExpressionMutationcontains bp# 1809-2217 of NM_010710.3PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK693
Plasmid#72382PurposeProduces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)DepositorInsertcysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationMutated alanine-260 to threoninePromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muEDC-Int3
Plasmid#59288PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the mouse Epidermal Differentiation Complex.DepositorInsertmFlg int-Intron 1
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Nanog(Y136A)-IP
Plasmid#62995PurposeExpresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).DepositorInsertMouse Nanog Y136A Mutant (Nanog Mouse)
UseTagsExpressionMammalianMutationChanged Tyrosine 136 (137 AA in Mouse Protein) to…PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
p14-9VNA5
Plasmid#50931PurposeExpresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.DepositorUseTagsVN tag and flag tagExpressionMammalianMutationAmino acids 96-100 of SRP14 changed to alaninePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
