We narrowed to 4,136 results for: pCMV mammalian

1. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

   Plasmid

   #221994

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF_PE2

   Plasmid

   #199267

   Purpose

   Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Expression

   Mammalian

   Mutation

   Δ40-903

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TadA-NG-CBE

   Plasmid

   #227420

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   TadA-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AID-NG-CBE

   Plasmid

   #227416

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   AID-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyCas9 PE2*-3HA

   Plasmid

   #169854

   Purpose

   Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-star-M-MLV-3HA
   

   Tags

   3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-SUMO-A'-l4-G'-IRES-EmGFP

   Plasmid

   #209127

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G'-IRES-EmGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SUMO-A'-l4-G-IRES-EmGFP

   Plasmid

   #209126

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G-IRES-EmGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBL649 CHYRON2 integration construct

   Plasmid

   #126446

   Purpose

   To integrate the CHYRON2 locus at AAVS1 in human cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON2 hgRNA
   pCMV-puro
   

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. YEE-NG-CBE

   Plasmid

   #227418

   Purpose

   C-to-T base editing in mammalian cells

   Depositor

   Yang Sun

   Article

   Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

   Insert

   rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mito NAD+ biosensor

    Plasmid

    #186791

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    mito NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. cytoplasmic NAD+ biosensor

    Plasmid

    #186787

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. nuclear NAD+ biosensor

    Plasmid

    #186789

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

    Plasmid

    #209296

    Purpose

    mitoBE expression vector, target mtRNR2 gene.

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

    Insert

    COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-MATR3

    Plasmid

    #32880

    Depositor

    Yossi Shiloh

    Article

    Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

    Insert

    MATR3 Matrin3 (MATR3 Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2.2

    Plasmid

    #34885

    Depositor

    Irvin Chen

    Article

    Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

    Insert

    Sindbis virus envelope protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    SGN UTR in E1 domain

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag Snail WT

    Plasmid

    #16218

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCiPS-NIFV

    Plasmid

    #110807

    Purpose

    Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

    Depositor

    David Russell

    Article

    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

    Insert

    Pol
    

    Expression

    Mammalian

    Mutation

    2 mutations in the DDE catalyic core motif of the…

    Promoter

    CMV

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2XMyc-LRRK2-3XKD

    Plasmid

    #25366

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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