We narrowed to 7,591 results for: Lif;

1. SspB-iRFP-DHPH-ITSN1

   Plasmid

   #176117

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK1520-LRRK2-G2019S-ng

   Plasmid

   #180436

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-pegRNA-SNCA-A53T

   Plasmid

   #181738

   Purpose

   Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBPK1520-SNCA-A53T-ng

   Plasmid

   #181739

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A53T (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AC27 pEGFPN1 anti-AMIGO-1 nanobody

   Plasmid

   #135226

   Purpose

   Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-AMIGO-1 (Mouse) recombinant llama nanobody AC27 (Amigo1 L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. HC89 pComb3xss anti-Homer1 nanobody

   Plasmid

   #134719

   Purpose

   Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HC89 (Homer1 L. glama (llama))
   

   Use

   Tags

   6xHis-HA

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

   Plasmid

   #161923

   Purpose

   To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting HSD17B11 exon 1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-MAFF

   Plasmid

   #192917

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of MAFF

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   MAFF (MAFF Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus-Bad-4E-pEGFP-C1

   Plasmid

   #166740

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

   Depositor

   David Andrews

   Article

   Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

   Insert

   Bad-4E (BAD Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   4 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBPK1520-HEK3-CTTins-ng

    Plasmid

    #180019

    Purpose

    Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U7

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLU-CMV-Flag-hATG5 E122D

    Plasmid

    #111804

    Purpose

    Mammalian Expression of hATG5

    Depositor

    Jun Hee Lee

    Article

    Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

    Insert

    hATG5 (ATG5 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E122D

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGTag-NLS-TagRFP-B-actin

    Plasmid

    #117806

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cntn2-Fc-His

    Plasmid

    #72066

    Purpose

    Expresses the extracellular region of the Contactin 2 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn2 (Cntn2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-SAP97 [K64/15R]

    Plasmid

    #128624

    Purpose

    Mammalian Expression Plasmid of anti-SAP97 (Rat). Derived from hybridoma K64/15.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-SAP97 (Rat) recombinant mouse monoclonal antibody (Dlg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGTag-eGFP-CAAX-B-actin

    Plasmid

    #117816

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    CAAX

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGTag-NLS-eGFP-B-actin

    Plasmid

    #117812

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.27_eSOX17

    Plasmid

    #195498

    Purpose

    Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Full distal endoderm enhancer region of SOX17 (SOX17 Human)
    

    Use

    Tags

    minP_luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.27_eSOX17.2

    Plasmid

    #195499

    Purpose

    Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Core distal endoderm enhancer region of SOX17 (SOX17 Human)
    

    Use

    Tags

    minP_luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AKAP1-EGFP-uTEV1

    Plasmid

    #171005

    Purpose

    HiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    AKAP1(tmd)-EGFP-uTEV1 (AKAP1 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Jam1-Fc-His

    Plasmid

    #72078

    Purpose

    Expresses the extracellular region of the JAM-1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Jam1 (F11r Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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