We narrowed to 9,978 results for: Met;

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LoxP3-Mb2-HA-mTFP1-2A (SO157)

Plasmid

#99615

Purpose

To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pERB127

Plasmid

#58283

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA

Tags

GFP and mCherry

Expression

Mammalian

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoD-NAD

Plasmid

#193826

Purpose

CMV overexpression of ChemoD-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoD-NAD

Expression

Mammalian

Mutation

ShadowG: T225R, HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT3(E338Q)

Plasmid

#164696

Purpose

Expression of PRMT3(E338Q) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

Insert

PRMT3 (PRMT3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E338Q

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mMaple3

Plasmid

#154912

Purpose

Expresses human PFAS-mMaple3 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Chan et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

PFAS (PFAS Human)

Tags

mMaple3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf3b

Plasmid

#158373

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3b

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-PTPN11-E76Q

Plasmid

#116642

Purpose

Lentiviral expression of PTPN11 E76Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Mutation

E76Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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hisSUMO-ZFP57

Plasmid

#102716

Purpose

Express DNA binding domain of human Zinc finger protein 57 in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

Zinc finger protein 57 (ZNF57 Human)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-PKN1 shRNA 4

Plasmid

#59978

Purpose

Knockdown of PKN1

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

PKN1 shRNA

Use

Retroviral

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPN11i1

Plasmid

#59612

Purpose

Expression of shRNA against human PTPN11

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPN11 (PTPN11 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
SI4-Jamc.2

Plasmid

#28216

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Jamc.2 mouse monoclonal antibody (jam3a Zebrafish)

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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ML2

Bacterial Strain

#61906

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
PTPN11 in pENTR

Plasmid

#216604

Purpose

gateway cloning donor vector containing PTPN11

Depositor

Douglas Allan, Christopher Loewen

Insert

PTPN11 (PTPN11 Human)

Use

Gateway Cloning

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMG81-MBP-t-M.MpeI-N374K-His

Plasmid

#197985

Purpose

Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

Depositor

Rahul Kohli

Article

Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

Insert

MBP-t-M.MpeI-N374K-His

Tags

His and MBP

Expression

Bacterial

Mutation

M.MpeI-N374K

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-ATP

Plasmid

#193819

Purpose

CMV overexpression of ChemoB-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-ATP

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pM115: CasRx control presgRNA

Plasmid

#166867

Purpose

U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

non-targeting presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-TMPRSS2

Plasmid

#158385

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

TMPRSS2 (TMPRSS2 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CRISPR sgSHMT2_1

Plasmid

#106308

Purpose

Express Cas9 and sgRNA targeting SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgSHMT1_1

Plasmid

#106311

Purpose

Express Cas9 and sgRNA targeting SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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GST-PRMT7 LDIG69AAAA

Plasmid

#61560

Purpose

Hs GST-PRMT7 LDIG(69-72)AAAA AdoMet binding deficient mutant

Depositor

Steven Clarke

Article

Feng et al J Biol Chem. 2014 Nov 21;289(47):32604-1

Insert

PRMT7 (PRMT7 Human)

Tags

GST

Expression

Bacterial

Mutation

LDIG 69-72 AAAA

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only