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pDV05.0_pEF1a-iRFP720

Plasmid

#200407

Purpose

Expresses iRFP720 constitutively in mammalian cells

Depositor

James Collins

Publication

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC33A1

Plasmid

#132264

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC33A1 (SLC33A1 Human)

Expression

Mammalian

Available since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pET24a-ncOGT-FL

Plasmid

#190821

Purpose

For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

Depositor

Gerald Hart

Publication

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for expression in E.coli

Promoter

T7

Available since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO-shSmad2

Plasmid

#37051

Depositor

Sam Thiagalingam

Publication

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad2 (SMAD2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
p6618 MSCV-IP N-HAonly WHSC2

Plasmid

#35014

Depositor

Peter Howley

Publication

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WHSC2 (NELFA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pTOX10

Plasmid

#127455

Purpose

Encodes YhaV toxin. Gent-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_RAD21_1

Plasmid

#64057

Purpose

Expresses gRNA against human RAD21 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC-GFPM2

Plasmid

#102791

Purpose

Expresses GFP under WT T7 promoter

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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hSUMO1 GFP

Plasmid

#118360

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Publication

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFUChW Parkin shRNA Mul1 shRNA

Plasmid

#78523

Purpose

Lentiviral vector encoding two shRNAs and mCherry reporter

Depositor

David Chan

Publication

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)

Use

Lentiviral and RNAi

Promoter

H1

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-MED27

Plasmid

#15424

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED27 (MED27 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMXs-EGFP-ERas-IP

Plasmid

#13826

Depositor

Shinya Yamanaka

Publication

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GFP-NPM NESM-NLS1D

Plasmid

#13288

Depositor

Xin Wang

Publication

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A, deletion of aa134-aa142

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 c-SRC (250-536) HA

Plasmid

#42209

Depositor

Robert Lefkowitz

Publication

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (250-536) HA (SRC Human)

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 250-536

Promoter

CMV

Available since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only
D28 Cortactin GFP

Plasmid

#26723

Depositor

Anna Huttenlocher

Publication

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion removed 28 amino acids (aa# 334-361) enc…

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PIK4CB

Plasmid

#20578

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIK4CB (PI4KB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCherryGFP-Inframe-noFSE

Plasmid

#177619

Purpose

Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control).

Depositor

Junjie Guo

Publication

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

None

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cyto_K20

Plasmid

#133862

Purpose

Expression of cytoplasmic Ribosome stalling reporter

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Cyto_K20

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Publication

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Expression

Bacterial

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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