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We narrowed to 25,508 results for: crispr

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Showing: 3341 - 3360 of 25508 results

  7. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid
    #208979
    Purpose
    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.
    Depositor
    Quanfu Ma
    Insert
    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208979-plasmid-map-sequence-id-418375

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  8. LentiCRISPRv2-ACTB-C4

    Plasmid
    #229849
    Purpose
    Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA for ACTB gene
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    229849-plasmid-map-sequence-id-462154

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  9. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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  11. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

    Plasmid
    #126480
    Purpose
    Expresses Cas9-GSAlinker-TdT in mammalian cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    GSA linker
    TdT (DNTT Human)
    Expression
    Mammalian
    Mutation
    Changed codons to reduce repetitive sequences.
    Promoter
    CMV
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126480-plasmid-map-sequence-id-246056

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  12. pFRT/TO/GFP-SCAF4 (CRISPR_resistant)

    Plasmid
    #122471
    Purpose
    Dox-inducible GFP (N-terminal tag) SCAF4 expression
    Depositor
    Jesper Svejstrup
    Publication
    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SCAF4 (SCAF4 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Synonymous mutations conferring resistance to gRN…
    Promoter
    CMV
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122471-plasmid-map-sequence-id-241041

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  13. pFRT/TO/GFP-SCAF8 (CRISPR_resistant)

    Plasmid
    #122472
    Purpose
    Dox-inducible GFP (N-terminal tag) SCAF8 expression
    Depositor
    Jesper Svejstrup
    Publication
    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SCAF8 (SCAF8 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Synonymous mutations conferring resistance to gRN…
    Promoter
    CMV
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122472-plasmid-map-sequence-id-237985

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  14. Px330-EGFP-LRRK2-CRISPR/Cas9

    Plasmid
    #180437
    Purpose
    Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells
    Depositor
    Frank Soldner
    Publication
    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA targeting LRRK2-G2019S (LRRK2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    180437-plasmid-map-sequence-id-355854

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  16. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86611-plasmid-map-sequence-id-168847

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  17. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187948
    Purpose
    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187948-plasmid-map-sequence-id-373701

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  20. pX330_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173205
    Purpose
    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Dual U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173205-plasmid-map-sequence-id-339509

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Showing: 3341 - 3360 of 25508 results