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EC10161

Plasmid

#154060

Purpose

Level 0.5 Golden Gate backbone, containing loxP sites.

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZE31-DsRed-ms2x2

Plasmid

#26248

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

ms2 binding sites (x2)

Expression

Bacterial

Available Since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA

Plasmid

#21302

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

lambdaN-HA

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV FLIP FF

Plasmid

#19744

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKCdelta.RNAi

Plasmid

#10819

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC delta RNAi (PRKCD Human)

Use

RNAi

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTurquoise2

Plasmid

#118453

Purpose

Overexpression of yeast codon-optimized mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV600

Plasmid

#110723

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Article

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

Plasmid

#92402

Purpose

Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Kalyviotis et al (2020) Behavioral and Neural Genetics of

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and No tag, cytosolic expression

Promoter

bactin2

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_Melt-37-mCherry

Plasmid

#234060

Purpose

Encodes a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

Melt-37-mCh

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBN396

Plasmid

#109326

Purpose

Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

Depositor

Peter Askjaer

Article

Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

Insert

mex-5 (mex-5 Nematode)

Expression

Worm

Promoter

mex-5

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRSII42K

Plasmid

#194523

Purpose

High copy vector backbone with G418 selectivity, contains MCS

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymVenus

Plasmid

#118454

Purpose

Overexpression of yeast codon-optimized mVenus

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-coSCFP3A

Plasmid

#104015

Purpose

Plasmid encoding coSCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coSCFP3A

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as SCFP3A (codon optimized …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAN3944

Plasmid

#74702

Purpose

Circuit plasmid: 0x1C

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x1C

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCsy4

Plasmid

#44252

Depositor

Adam Arkin, Stanley Qi

Article

Qi et al Nat Biotechnol. 2012 Oct;30(10):1002-6.

Insert

csy4 gene

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLtetO-1

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:D70E

Plasmid

#79164

Purpose

Expresses constitutively active WspR D70E under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Mutation

D70E

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pJMH143

Plasmid

#186646

Purpose

aadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Jacob M. et al bioRxiv

Insert

aadA.co-TaadA-PJ23117-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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