We narrowed to 8,661 results for: pcas

1. gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

   Plasmid

   #196845

   Purpose

   backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-AnkG ZU5in-3xHA

   Plasmid

   #213730

   Purpose

   Mammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   AnkG ZU5in (Ank3 Mouse)
   

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpCas9HF1-P2A-puro

   Plasmid

   #214357

   Purpose

   Expresses SpCas9HF1 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9HF1 and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/Q926A

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-AnkB ZU5ex-3xHA

   Plasmid

   #213732

   Purpose

   Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   AnkB ZU5ex (Ank2 Mouse)
   

   Expression

   Mammalian

   Available Since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-AnkG ZU5ex-3xHA

   Plasmid

   #213731

   Purpose

   Mammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   AnkG ZU5ex (Ank3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaUra3-CUbo(K48R)-yeGFP

   Plasmid

   #212772

   Purpose

   C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCaUra3-CUbo(K63R)-yeGFP

   Plasmid

   #212773

   Purpose

   C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with CaUra3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

   Plasmid

   #211821

   Purpose

   PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
   

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter, U6 promoter and hPGK pr…

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGIG-FLAG-SHTN1LRRR>GGG

   Plasmid

   #209570

   Purpose

   Mammalian expression of Shtn1 long isoform with RG mutation

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)
   

   Expression

   Mammalian

   Mutation

   RRR to GGG

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cav2.2 BBS exon37a pCAGGS

    Plasmid

    #206086

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Promoter

    CMV/B-actin

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid

    #206082

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid

    #206081

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    E314A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-HypaSpCas9-NLS-6xHis

    Plasmid

    #207384

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP Cav2.2 HA L702 P pCAGGS

    Plasmid

    #206055

    Purpose

    expression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional

    Depositor

    Annette Dolphin

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L702P

    Promoter

    CMV/B-actin

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-B-HeFSpCas9-NLS-6xHis

    Plasmid

    #207392

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-HeFSpCas9-NLS-6xHis

    Plasmid

    #207391

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-evoSpCas9-NLS-6xHis

    Plasmid

    #207389

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid

    #206096

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV/B-actin

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid

    #206936

    Purpose

    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

    Depositor

    Markus Grompe

    Insert

    spCas9
    

    Use

    AAV

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    MP-84

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-FIP200 (1-640)-GST

    Plasmid

    #203544

    Purpose

    FIP200 NTD-MBP mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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