We narrowed to 7,043 results for: plasmid dna

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QSOX1-luciferase

Plasmid

#113352

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

QSOX1-enhancer (QSOX1 Human)

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSCL.180

Plasmid

#185001

Purpose

Express -Eco1 AAVS1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

AAVS1 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ATP9A-luciferase

Plasmid

#113354

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ATP9A-enhancer (ATP9A Human)

Use

Luciferase

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-miR-34 MT

Plasmid

#78259

Purpose

psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

Depositor

Joanne Weidhaas

Article

Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

Insert

mutated miR-34 target site

Use

Luciferase; Microrna activity

Expression

Mammalian

Mutation

nucleotides 2-5 and 10-11 changed from CCGT to GG…

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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SunTagCACTA1g2-22aa-TET1cd

Plasmid

#106437

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_KMT2B

Plasmid

#101070

Purpose

Donor vector for 3' FLAG tag of human KMT2B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KMT2B homology arms (KMT2B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pZJQIBEBT003

Plasmid

#140053

Purpose

The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

Depositor

Jia Zhang

Article

Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

Insert

eMutS-L157C-G233C-CBM-EGFP

Tags

His-Tag

Expression

Bacterial

Mutation

L157C, G233C

Promoter

T7

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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sTR060

Plasmid

#177370

Purpose

Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

Trigger RNA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gLacZ.Cas9-T2A-GFP

Plasmid

#170361

Purpose

Negative control plasmid used for Crispr/cas9 based disruption.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Promoter

U6

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFETCh_ARID4B

Plasmid

#101072

Purpose

Donor vector for 3' FLAG tag of human ARID4B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID4B homology arms (ARID4B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_RERE

Plasmid

#101069

Purpose

Donor vector for 3' FLAG tag of human RERE

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RERE homology arms (RERE Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_RERE_1

Plasmid

#101073

Purpose

Encodes gRNA for 3' target of human RERE

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against RERE (RERE Human)

Use

CRISPR

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-TAF1-A

Plasmid

#247346

Purpose

Expresses SpCas9 and a sgRNA targeting the human TAF1-A loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1 (TAF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-Dox-AIO-TetOn_ETV2_Down-Tandem

Plasmid

#241393

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ETV2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-ETV2; rtTA-T2A-mTagBFP2 (ETV2 Human)

Tags

NLS

Expression

Mammalian

Promoter

TRE; CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold

Plasmid

#226933

Purpose

Encoding Nme2ABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nNme2Cas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold

Plasmid

#226935

Purpose

Encoding SaKKHABE8e and sgRNA cassette on a single AAV

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

TadA8e, nSaKKHCas9

Use

AAV

Promoter

EFS

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA201

Plasmid

#216026

Purpose

Fragmid fragment: (Cas protein) firefly luciferase

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Fluc_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA156

Plasmid

#216022

Purpose

Fragmid fragment: (Cas protein) Nanobody binds ALFA tag

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

NbALFA_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA349

Plasmid

#216032

Purpose

Fragmid fragment: (Cas protein) Renilla luciferase

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Rluc_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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