We narrowed to 6,064 results for: ado

1. EF1a_KLF11_P2A_Hygro_Barcode

   Plasmid

   #120497

   Purpose

   Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF11 (KLF11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_HOXA11_P2A_Hygro_Barcode

   Plasmid

   #120453

   Purpose

   Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HOXA11 (HOXA11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Ctdnep1_D67E-2xFlag

   Plasmid

   #196519

   Purpose

   Expression of Ctdnep1_D67E-2xFlag

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   D67E mutation (phosphastase dead)

   Promoter

   CMV promoter; T7 promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1+CeGFP-Ctdnep1WTsiR

   Plasmid

   #196517

   Purpose

   Expression of Ctdnep1WT-GFP

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1WTsiR (CTDNEP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Silent mutations for siRNA resistance

   Promoter

   CMV promoter; T7 promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-HF1RA-P2A-Puro

   Plasmid

   #110853

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_HOXB6_P2A_Hygro_Barcode

   Plasmid

   #120454

   Purpose

   Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPCX2-HA-GATA3 C1(259-443)

   Plasmid

   #89832

   Purpose

   Express GATA3 C1 in mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Gata3 (Gata3 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   C1 (AA 259-443)

   Promoter

   CMV

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a_POU5F1_P2A_Hygro_Barcode

   Plasmid

   #120474

   Purpose

   Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP Kind2 S181E

   Plasmid

   #105312

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S181E

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP Kind2 S181A

    Plasmid

    #105307

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF1-MCS2-EF1-Puro-RGS7

    Plasmid

    #113728

    Purpose

    Express RGS7 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-BE3-P2A-GFP-PGK-Puro

    Plasmid

    #110867

    Purpose

    Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-VQRRA-P2A-Puro

    Plasmid

    #110851

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

    Plasmid

    #184406

    Purpose

    yeast surface display of the SARS-CoV-2 Beta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    K417N+E484K+N501Y

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti PA-mCit-NL

    Plasmid

    #113452

    Purpose

    Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBbC

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-HF1-PGK-Puro

    Plasmid

    #110857

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EF1a_HOXA1_P2A_Hygro_Barcode

    Plasmid

    #120451

    Purpose

    Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HOXA1 (HOXA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-VQRRA-PGK-Puro

    Plasmid

    #110858

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-Basic-Fascin-Promoter (-402-0)

    Plasmid

    #89823

    Purpose

    Promoter luciferase assay

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Fascin1 (FSCN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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