We narrowed to 3,644 results for: ORF1

Showing: 3361 - 3380 of 3644 results

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pAG416GALL-SARS-CoV-2_3CL

Plasmid

#203496

Purpose

Expresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-MERS-CoV_PLP

Plasmid

#203508

Purpose

Expresses MERS-CoV papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

MERS-CoV PLP (orf1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-SARS-CoV_3CL

Plasmid

#203495

Purpose

Expresses SARS-CoV 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-Bat-CoV-HKU4_3CL

Plasmid

#201943

Purpose

Expresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-CoV HKU4 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-NL63_3CL

Plasmid

#201938

Purpose

Expresses HCoV-NL63 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-NL63 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCoV-NL63_PLP2

Plasmid

#203502

Purpose

Expresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-NL63 PLP2 (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCoV-OC43_3CL

Plasmid

#203503

Purpose

Expresses HCoV-OC43 3CL protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-OC43 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-ΔN-CHMP4B(S184C)

Plasmid

#180026

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

S184C

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

Plasmid

#198765

Purpose

expression of hOCT4 mutants

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 236D (POU5F1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S236D

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-WFIKKN1-COMP5AP-AviTag-9xHis

Plasmid

#157469

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

WFIKKN1 (WFIKKN1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp7 (SARS-CoV-2)

Plasmid

#169178

Purpose

Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7 (ORF1ab SARS-CoV-2, Synthetic)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1c_nsp8 (SARS-CoV-2)

Plasmid

#169179

Purpose

Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Tags

untagged

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-TOM20MTS-DDRGK1-dTM-HA

Plasmid

#139861

Purpose

Lentiviral expression of TOM20MTS-DDRGK1-dTM-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

TOM20-MTS-DDRGK1-dTM (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

recombinant fusion of mitochondria-targeting sign…

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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IDNK gRNA (BRDN0001146514)

Plasmid

#75865

Purpose

3rd generation lentiviral gRNA plasmid targeting human IDNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IDNK (C9orf103 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IDNK gRNA (BRDN0001147773)

Plasmid

#75866

Purpose

3rd generation lentiviral gRNA plasmid targeting human IDNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IDNK (C9orf103 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IDNK gRNA (BRDN0001148428)

Plasmid

#75867

Purpose

3rd generation lentiviral gRNA plasmid targeting human IDNK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IDNK (C9orf103 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLIRP_WT

Plasmid

#82288

Purpose

Gateway Donor vector containing SLIRP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLIRP (SLIRP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

Plasmid

#89992

Purpose

Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)

Use

CRISPR

Promoter

none

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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