We narrowed to 49,149 results for: ple

1. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

   Plasmid

   #163644

   Purpose

   Makes a lentivirus that expresses mNeonGreen and Cas9.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   mNeonGreen-P2A-Cas9
   

   Use

   Lentiviral

   Tags

   mNeonGreen-P2A

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

   Plasmid

   #159802

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

   Plasmid

   #138368

   Purpose

   Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP

   Plasmid

   #69050

   Purpose

   lentiviral expression of IKZF3 with Q147H mutation and FLAG tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   Q147H

   Promoter

   CAG

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

   Plasmid

   #73455

   Purpose

   Express human middle part (Q513-E784) Plekhm1 in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   CBP tag, HA tag, and strep tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

   Plasmid

   #166134

   Purpose

   This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

   Insert

   hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

   Plasmid

   #167199

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R S364L
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

   Plasmid

   #167200

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R D421N
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

    Plasmid

    #167198

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E294K
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

    Plasmid

    #167197

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R M173I
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

    Plasmid

    #167196

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E144K
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

    Plasmid

    #130678

    Purpose

    Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_F489del

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

    Plasmid

    #130691

    Purpose

    Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG and 3xHA

    Expression

    Mammalian

    Mutation

    E653_G1960del

    Promoter

    CMV

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

    Plasmid

    #130690

    Purpose

    Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    1M_T1522delinsM, G1720_Y1846del

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. plenti-UbcP-Myc-YWAA-pGK-HYG

    Plasmid

    #107377

    Purpose

    Lentiviral expression of myc-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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