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pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Plasmid

#179106

Purpose

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Use

CRISPR

Expression

Mammalian

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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hAur-A

Plasmid

#8510

Depositor

Joan Ruderman

Publication

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hAur-A (AURKA Human)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Plasmid

#179116

Purpose

Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site for in-frame fusion

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-Rab5CA(Q79L)

Plasmid

#35138

Depositor

Sergio Grinstein

Publication

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5a (RAB5A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Constitutively Active- Q79L

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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tdTomato-N1

Plasmid

#54642

Purpose

Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Publication

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Plasmid

#179115

Purpose

Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PPRE-H2B-eGFP

Plasmid

#84393

Purpose

Fluorescent reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Publication

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PPARG response element (PPARG Human)

Tags

eGFP

Expression

Mammalian

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLV-EF1a-IRES-Blast

Plasmid

#85133

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

61 Articles

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pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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BFP-Sec61 beta

Plasmid

#49154

Purpose

expression of Sec61 beta fused to TagBFP, used as a general ER marker

Depositor

Gia Voeltz

Publication

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec61 beta (SEC61B Human)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Publication

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-EF1a-IRES-Hygro

Plasmid

#85134

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

50 Articles

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mCherry-Rab5DN(S34N)

Plasmid

#35139

Depositor

Sergio Grinstein

Publication

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab5a (RAB5A Human)

Tags

mCherry

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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PPRE-pNL1.3

Plasmid

#84394

Purpose

Secreted Luciferase reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Publication

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PPARG response element (PPARG Human)

Tags

secNluc

Expression

Mammalian

Promoter

PPRE

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-hsyn-GRAB_rDA1m

Plasmid

#140556

Purpose

Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

Depositor

Yulong Li

Publication

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has service

AAV9

Insert

Red fluorescent DA sensor GRAB_rDA1m (medium affinity)

Use

AAV

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.hSynapsin.SF-iGluSnFR.A184S

Plasmid

#106174

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has service

AAV1 and AAV9

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

hSynapsin

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

1 Data Hub Report

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pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Publication

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SC35 (SRSF2 Human)

Tags

his and myc

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pT3-EF1a-Bmi1

Plasmid

#31783

Depositor

Xin Chen

Publication

Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bmi1 (BMI1 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLV-EF1a-IRES-Neo

Plasmid

#85139

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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