We narrowed to 9,329 results for: pho

1. EGFP-SopB(33-554)

   Plasmid

   #183661

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EGFP-SopB(33-520)

   Plasmid

   #183662

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 33-520

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-SopB(68-554)

   Plasmid

   #183663

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 68-554

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pESC-LEU-IpgD(C439S)

   Plasmid

   #183673

   Purpose

   Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   IpgD
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C439S

   Promoter

   GAL1

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SopB(C460S)-EGFP

   Plasmid

   #183656

   Purpose

   C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EGFP-SopB(C460S)

   Plasmid

   #183658

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C460S

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human SHIP2

   Plasmid

   #124645

   Purpose

   Expresses HIS tagged human SHIP2 in COS-7/HEK cells

   Depositor

   Christophe Erneux

   Article

   Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

   Insert

   INPPL1 (INPPL1 Human)
   

   Use

   Tags

   His Xpress (N-terminal)

   Expression

   Mammalian

   Mutation

   coding sequence starts at amino acid 18 as calcul…

   Promoter

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. His6-SMT3-SopB(C460S)

   Plasmid

   #183677

   Purpose

   Recombinant Salmonella Typhimurium SopB (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   C460S

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-SopB(172-554)

   Plasmid

   #183664

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Amino acids 172-554

   Promoter

   CMV

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-SopB(211-554)

    Plasmid

    #183665

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 211-554

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His6-EGFP-cPHx1

    Plasmid

    #183674

    Purpose

    PtdIns(3,4)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 169-329

    Promoter

    T7

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NES-EGFP-PH-BTKx2

    Plasmid

    #183652

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 2-174 and 2-170

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NES-mCherry-PH-BTKx2

    Plasmid

    #183654

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-174 and 2-170

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NES-EGFP-PH-BTKx1

    Plasmid

    #183651

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 2-170

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUG36-NES-EGFP-cPHx3

    Plasmid

    #183668

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Frog, Human)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUG34-NES-EGFP-cPHx3

    Plasmid

    #183669

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Frog, Human)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NES-mCherry-PH-BTKx1

    Plasmid

    #183653

    Purpose

    PtdIns(3,4,5)P3 biosensor

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    BTK (BTK Frog, Human)
    

    Use

    Tags

    X. laevis map2k1.L(32-44)-mCherry

    Expression

    Mammalian

    Mutation

    Amino acids 2-170

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-N-TEV-Twin-Strep

    Plasmid

    #222014

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

    Depositor

    Chiaki Murakami

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcGFP-C1-DGKd2

    Plasmid

    #224897

    Purpose

    Express N-terminal mCherry fused to human diacylglycerol kinase delta isoform 2 in mammalian cells

    Depositor

    Chiaki Murakami

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-Sfp R4-4 mutant

    Plasmid

    #173491

    Purpose

    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

    Depositor

    Jun Yin

    Article

    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

    Insert

    Sfp-R44
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge