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YCe1715 HC_Kan_RFP-p23

Plasmid

#100633

Purpose

EMMA toolkit part-entry vector position 23

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1721 HC_Kan_RFP-p25

Plasmid

#100635

Purpose

EMMA toolkit part-entry vector position 25

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1693 HC_Kan_RFP-p1

Plasmid

#100609

Purpose

EMMA toolkit part-entry vector position 1

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1500 HC_Kan_RFP-p2

Plasmid

#100610

Purpose

EMMA toolkit part-entry vector position 2

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1502 HC_Kan_RFP-p3

Plasmid

#100611

Purpose

EMMA toolkit part-entry vector position 3

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1505 HC_Kan_RFP-p4

Plasmid

#100612

Purpose

EMMA toolkit part-entry vector position 4

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1506 HC_Kan_RFP-p5

Plasmid

#100613

Purpose

EMMA toolkit part-entry vector position 5

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-PTS1

Plasmid

#44588

Purpose

Gateway entry vector for Citrine fluorescent protein with a peroxisome localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-KstR

Plasmid

#51179

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

KstR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-CampR

Plasmid

#51162

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

CampR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGST1-yVps27-UIM1

Plasmid

#44426

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Vps27-UIM1 (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
C119

Plasmid

#174354

Purpose

CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

gRNA sequence targeting human PABPC1 near stop codon region

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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C205

Plasmid

#174359

Purpose

For expressing siRNA-resistant frog ePAB in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Availability

Academic Institutions and Nonprofits only

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hPGK-rtTA3

Plasmid

#195883

Purpose

Lentiviral construct of hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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UIMLx2

Plasmid

#174888

Purpose

UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

Depositor

Peter Kaiser

Article

Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

Insert

Met4 UIML

Tags

Avi-6xHis-Smt3

Expression

Bacterial

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Article

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAB50

Plasmid

#101678

Purpose

mCherry under T7 promoter control

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

mCherry under T7 promoter control

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB102-Citrine-NLS

Plasmid

#79136

Purpose

Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Tags

NLS (Nuclear Localization Signal)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4-PKCZeta WT His tagged

Plasmid

#24609

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (PRKCZ Human)

Tags

His

Expression

Mammalian

Available Since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits