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We narrowed to 25,508 results for: crispr

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Showing: 3381 - 3400 of 25508 results

  11. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  12. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187951-plasmid-map-sequence-id-373504

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  13. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Publication
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Mustard Weed, S. aureus, Synthetic)
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

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  14. pCRISPR3_BC2

    Plasmid
    #191857
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Publication
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191857-plasmid-map-sequence-id-381604

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Showing: 3381 - 3400 of 25508 results