We narrowed to 30,657 results for: Nes

1. pLX302 LacZ-V5 puro

   Plasmid

   #98579

   Purpose

   Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   2x V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Blue_CaMBI

   Plasmid

   #124097

   Purpose

   Blue calcium-modulated bioluminescent indicator

   Depositor

   Michael Lin

   Article

   Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

   Insert

   Blue CaMBI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRFLIP38

   Plasmid

   #65516

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5'Entry-lck-5.5kb

   Plasmid

   #58890

   Purpose

   provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
   

   Use

   Gateway 5' entry clone

   Tags

   Expression

   Mutation

   mutated single nucleotide (T-->C, 13th nucleot…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Green_CaMBI

   Plasmid

   #124096

   Purpose

   Green calcium-modulated bioluminescent indicator

   Depositor

   Michael Lin

   Article

   Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

   Insert

   Green CaMBI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEP109E

   Plasmid

   #97395

   Purpose

   binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   mCherry-sfGFP11
   

   Use

   Tags

   cytosol

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GeNL(Ca2+)_60/pcDNA3

   Plasmid

   #85207

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_60
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSGV Hu Acceptor PGK-NGFR

   Plasmid

   #64270

   Purpose

   Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

   Depositor

   David Ott

   Article

   Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUPD3

   Plasmid

   #118043

   Purpose

   GoldenBraid2.0 Universal Part Domesticator #3

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A_dCas9-1x2

    Plasmid

    #63596

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-TarHom-Tol2-kanaR-cryaa-dsRed

    Plasmid

    #74152

    Purpose

    BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRed

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    Tol2-kanaR-cryaa-dsRed
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. OeNL_pRSETb

    Plasmid

    #89529

    Purpose

    Orange color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMaCTag-07

    Plasmid

    #124786

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ReNL-H2B_pcDNA3

    Plasmid

    #89534

    Purpose

    Red color variant of bright luminescent protein, fused to histone 2B

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    ReNL
    

    Use

    Tags

    histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-cdc2 (716)

    Plasmid

    #61840

    Purpose

    mammalian expression of cdc2

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    cdc2 (CDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLIK 3XFLAG-EGFP-NLS hygro

    Plasmid

    #87781

    Purpose

    Inducible expression of EGFP with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Nano-lantern(ATP1)/pcDNA3

    Plasmid

    #51992

    Purpose

    mammalian expression of a Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Cox8x2-CeNL_pcDNA3

    Plasmid

    #89513

    Purpose

    Cyan color variant of bright luminescent protein, targeted to the Mitochondria

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    CoxVIII X2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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