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pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)

Plasmid

#20174

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (K83A,D84N,G86D) in the EF hand of ST…

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pPCK

Plasmid

#133128

Purpose

a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

PEP-carboxykinase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgMYC

Plasmid

#125770

Purpose

constitutive expression of a guide RNA targeting human MYC (CRISPR positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgMYC (MYC Human)

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHUM

Plasmid

#64170

Purpose

harbours marker genes HIS3, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-H10-mCherry

Plasmid

#66531

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H10

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H10

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pHYDuet183

Plasmid

#41685

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

PhoRadA intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

Plasmid

#246755

Purpose

RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

Use

Synthetic Biology

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1s-ST-WPRE

Plasmid

#214968

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1s-ST

Use

AAV

Promoter

human Synapsin

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA M66I

Plasmid

#149687

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue Met 66 to Iso (CA M66I)

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the TM287/288 nanobody Nb_TM#2

Plasmid

#132698

Purpose

expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

nanobody Nb_TM#2

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SLF091: Lenti-CaMKIIa-eTsChR-eGFP

Plasmid

#112281

Purpose

For lentiviral or direct expression of eTsChR in excitatory neurons

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

eTsChR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLSLGUS

Plasmid

#51517

Purpose

pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GUS
3kb filler sequence

Use

Plant t-dna plasmid

Tags

NLS and flag

Promoter

virion-sense LIR and 2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-PEPD

Plasmid

#31328

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

peptidase D (PEPD Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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RGS16

Plasmid

#39140

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RGS16 (RGS16 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pSKDuet26

Plasmid

#41689

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

Tvo VMA intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pDUP 4-5

Plasmid

#23023

Depositor

Douglas Black

Article

Modafferi et al Mol Cell Biol. 1997 Nov . 17(11):6537-45

Insert

c-src (Src Mouse)

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pQTEV-MTPN

Plasmid

#34829

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

myotrophin (MTPN Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pMMRSF1-16

Plasmid

#20177

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

src homology domain 3 (CRK Human)

Tags

His and NpuDnaEC16

Expression

Bacterial

Available Since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
PB-mCherry-DMDEx23-eGFP

Plasmid

#211366

Purpose

Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

Depositor

Ulrich Lächelt

Article

Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

Insert

mCherry-DMDEx23-eGFP

Expression

Mammalian

Mutation

mCherry interrupted by mdx dystrophin exon 23 bet…

Promoter

CAG

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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