We narrowed to 24,891 results for: Spr

1. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ133D2.0

   Plasmid

   #99894

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ131D2.0

   Plasmid

   #100044

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPN021

   Plasmid

   #91667

   Purpose

   Express sgRNA targeting human SHANK3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   SHANK3 (SHANK3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS316-RGR-GFP-mm

   Plasmid

   #51059

   Purpose

   Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NAX_CAGGS_CAS9_m4_KRAB_EBFP2

   Plasmid

   #167873

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTX208

   Plasmid

   #89268

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsYSA-gRNA01 and OsYSA-gRNA02
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB3043(gRNA XI-1)

   Plasmid

   #73285

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-1

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB3049(gRNA XII-4)

   Plasmid

   #73291

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GG-EBNA-TdT-guide1-PGK-Puro

    Plasmid

    #102903

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    TdT-guide1-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS316-RGR-GFP-mHDV

    Plasmid

    #51058

    Purpose

    Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX458_MBD1_iso1_2

    Plasmid

    #86304

    Purpose

    Encodes gRNA for 3' target of human MBD1_iso1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against MBD1_iso1 (MBD1 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX458_GATAD2A_2

    Plasmid

    #86334

    Purpose

    Encodes gRNA for 3' target of human GATAD2A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against GATAD2A (GATAD2A Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX458_GATAD2A_1

    Plasmid

    #86333

    Purpose

    Encodes gRNA for 3' target of human GATAD2A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against GATAD2A (GATAD2A Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

    Plasmid

    #149656

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPN144

    Plasmid

    #91673

    Purpose

    Express sgRNA targeting human TBC1D5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TBC1D5 (TBC1D5 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPN145

    Plasmid

    #91674

    Purpose

    Express sgRNA targeting human TBC1D5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TBC1D5 (TBC1D5 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPN216

    Plasmid

    #91675

    Purpose

    Express sgRNA targeting human TLE1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TLE1 (TLE1 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RFN_EGFP_47

    Plasmid

    #60071

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pPN023

    Plasmid

    #91671

    Purpose

    Express sgRNA targeting human SYNGAP1

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SYNGAP1 (SYNGAP1 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge