We narrowed to 11,554 results for: Vars;
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Plasmid#138974PurposeLight-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
UseTagsExpressionMutationPromoterAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIs8-4b-SaDAH::G226D
Plasmid#140130PurposeExpresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase G226D variant from Sinomenium acutum
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationchanged Gly226 to Asp226PromoterT7Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 539
Plasmid#138078PurposeControl vector with no degradation tag to compare against various degradation tag variantsDepositorInsertJ23104-mCherry::sbfI- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable SinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-HypaCAS9-pA
Plasmid#131467PurposeExpresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized HypaCas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-nolinker-GFP
Plasmid#108579PurposeExpresses PKAcs-GFP fusion in mammalian cellsDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-G12X-GFP
Plasmid#108588PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω2
Plasmid#101705PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22Ω1
Plasmid#101706PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α2
Plasmid#101707PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YORWΔ22α1
Plasmid#101708PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω2
Plasmid#101709PurposeGoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15α2
Plasmid#101711PurposeGoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD-MAM33MTS (12)
Plasmid#101693PurposeMAM33MTS in domestication vectorDepositorInsertCCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)from S. cerevisiae Mitochondrial acidic protein (YIL070C)]-AATG
UseYeast expressionTagsExpressionMutationPromoterAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AC104650.1-1/1
Plasmid#91541PurposeProtein expression and purification of human SH3 domain construct AC104650.1-1/1DepositorInsertAC104650.1-1/1
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AC093799.1-1/1
Plasmid#91495PurposeProtein expression and purification of human SH3 domain construct AC093799.1-1/1DepositorInsertAC093799.1-1/1
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_OTOR-2/2
Plasmid#91382PurposeProtein expression and purification of human SH3 domain construct OTOR-2/2DepositorInsertOTOR-2/2
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_OTOR-1/2
Plasmid#91328PurposeProtein expression and purification of human SH3 domain construct OTOR-1/2DepositorInsertOTOR-1/2
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RIMBP3B-1/1
Plasmid#91299PurposeProtein expression and purification of human SH3 domain construct RIMBP3B-1/1DepositorInsertRIMBP3B-1/1
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LOC440461-1/1
Plasmid#91309PurposeProtein expression and purification of human SH3 domain construct LOC440461-1/1DepositorInsertLOC440461-1/1
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_C1orf168-1/1
Plasmid#91295PurposeProtein expression and purification of human SH3 domain construct C1orf168-1/1DepositorInsertC1orf168-1/1
UseTagsExpressionBacterialMutationPromoterAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only