We narrowed to 8,347 results for: construct

1. pTH858-gst-optec V156E

   Plasmid

   #89592

   Purpose

   Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed valine 156 to glutamic acid

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH859-gst-optec Y172M

   Plasmid

   #89593

   Purpose

   Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed tyrosine 172 to methionine

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E-GFP-HA no-pA

   Plasmid

   #82589

   Purpose

   3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   GFP-HA (no pA)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRPa-c6MYCn

   Plasmid

   #69242

   Purpose

   Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Tags

   cMyc

   Expression

   Mutation

   Promoter

   RRNA promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRP-c6MYCn

   Plasmid

   #69243

   Purpose

   Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Tags

   cMyc

   Expression

   Mutation

   Promoter

   RRNA promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRPa-iSL(MCS3+4)

   Plasmid

   #69246

   Purpose

   Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

   Depositor

   David Horn

   Article

   Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

   Type

   Empty backbone

   Use

   Expression in trypanosoma brucei

   Tags

   Expression

   Mutation

   Promoter

   RRNA promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHJ33

   Plasmid

   #48362

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDS69

   Plasmid

   #48367

   Purpose

   FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
   

   Use

   Cre/Lox; Knockout in t. brucei

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNH066

   Plasmid

   #42162

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   N-TAP Tag-RT-::mTFP1
   

   Use

   Tags

   TAP-TAg

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNH094

    Plasmid

    #42167

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1-RT-::[(G4S)3]::C-TAP Tag
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNH090

    Plasmid

    #42166

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1::[(G4S)3]::C-TAP Tag
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNH009

    Plasmid

    #42146

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    N-TAP-tag::mTFP1
    

    Use

    Tags

    TAP-Tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAL13

    Plasmid

    #37186

    Depositor

    Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Type

    Empty backbone

    Use

    Tags

    FokI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHD8

    Plasmid

    #30981

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat HD8
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNG5

    Plasmid

    #30989

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NG5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNI6

    Plasmid

    #31001

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NI6
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNN1

    Plasmid

    #31007

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NN1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNN3

    Plasmid

    #31009

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NN3
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNN4

    Plasmid

    #31010

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NN4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNN5

    Plasmid

    #31011

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NN5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge