We narrowed to 15,788 results for: LEA

1. pL0M-S-mClover3-EC18157

   Plasmid

   #137076

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p32m3C Trx-CC

   Plasmid

   #196475

   Purpose

   Protein expression of Thiolredoxin-3C cleavage site-CarHCC

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   Trx-CC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-delta8.2R

   Plasmid

   #89706

   Purpose

   Donor plasmid for HIV-1 env- integration into human AAVS1 locus

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

   Insert

   AAVS1-delta8.2R
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-perox-mCer

   Plasmid

   #185603

   Purpose

   Lentiviral construct for expression of mCerulean targeted to peroxisomes

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   perox-mCer
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30a-YedK

   Plasmid

   #86884

   Purpose

   Bacterial expression of E. coli SRAP ortholog YedK

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   YedK (yedK Eschericia coli)
   

   Use

   Tags

   6xHis, HA tag, and S-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-mCherry-FKBP-TEVp

   Plasmid

   #178867

   Purpose

   Mammalian expression of mCherry-FKBP-TEVprotease(Δ220-242)

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   mCherry-FKBP-TEVprotease(Δ220-242)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FLAG-Pol-II 15

   Plasmid

   #35177

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains the C-terminal repeats 16-52

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pExodus

   Plasmid

   #39991

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFREE2-xCas9-sgRNA

   Plasmid

   #179579

   Purpose

   Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   xCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-LacI-polylinker

    Plasmid

    #179271

    Purpose

    Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

    Depositor

    Lienhard Schmitz

    Article

    Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

    Type

    Empty backbone

    Use

    Tags

    GFP and LacI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

    Plasmid

    #52478

    Purpose

    Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    EGFP
    nuclear mTagBFP
    Synaptophysin-TagRFP-T
    

    Use

    Rabies virus

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT316_pattB-pCA-GFP-pA

    Plasmid

    #52548

    Purpose

    for Phic31 integrase-mediated transgene integration

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSHS293

    Plasmid

    #87348

    Purpose

    D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/D435C/C574S/E945C

    Promoter

    T7

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGM-ENO2-mCherry-YFP

    Plasmid

    #180570

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-PB1-AG-FRB

    Plasmid

    #178862

    Purpose

    Mammalian expression of PB1-AzamiGreen-FRB

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LDB107

    Plasmid

    #185423

    Purpose

    NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

    Depositor

    Ken Belanger, Laura Davis

    Article

    Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

    Insert

    NUP1
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pH1-shOPA1-pSyn-tdtomato

    Plasmid

    #206316

    Purpose

    Knocking-down OPA1 in mouse cells

    Depositor

    Yusuke Hirabayashi

    Article

    Suga et al PLoS Biol. 2023 Aug 31;21(8):e3002246. d

    Insert

    shRNA against mouse OPA1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGM-ENO2-YFP-mCherry

    Plasmid

    #180569

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCLX-UBI-Tred

    Plasmid

    #27246

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    TurboRFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMINTC3GH (Apr)

    Plasmid

    #110078

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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