We narrowed to 15,991 results for: LEA

1. pFREE2-xCas9-sgRNA

   Plasmid

   #179579

   Purpose

   Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   xCas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

   Plasmid

   #52478

   Purpose

   Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

   Inserts

   EGFP
   nuclear mTagBFP
   Synaptophysin-TagRFP-T
   

   Use

   Rabies virus

   Tags

   TagRFP-T

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT316_pattB-pCA-GFP-pA

   Plasmid

   #52548

   Purpose

   for Phic31 integrase-mediated transgene integration

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attB (full length)
   GFP
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-LacI-polylinker

   Plasmid

   #179271

   Purpose

   Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

   Depositor

   Lienhard Schmitz

   Article

   Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

   Type

   Empty backbone

   Tags

   GFP and LacI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCLX-UBI-Tred

   Plasmid

   #27246

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   TurboRFP
   

   Use

   Lentiviral

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGM-ENO2-mCherry-YFP

   Plasmid

   #180570

   Purpose

   Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

   Depositor

   Joel McManus

   Article

   May et al Elife. 2023 May 25;12:e69611. doi: 10.75

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PB1-AG-FRB

   Plasmid

   #178862

   Purpose

   Mammalian expression of PB1-AzamiGreen-FRB

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen-FRB
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGM-ENO2-YFP-mCherry

   Plasmid

   #180569

   Purpose

   Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

   Depositor

   Joel McManus

   Article

   May et al Elife. 2023 May 25;12:e69611. doi: 10.75

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMINTC3GH (Apr)

    Plasmid

    #110078

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-Pol-II CC

    Plasmid

    #35180

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains C-terminal domain repeats (1-22)X2

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. LDB107

    Plasmid

    #185423

    Purpose

    NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

    Depositor

    Ken Belanger, Laura Davis

    Article

    Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

    Insert

    NUP1
    

    Tags

    HA

    Expression

    Yeast

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH1-shOPA1-pSyn-tdtomato

    Plasmid

    #206316

    Purpose

    Knocking-down OPA1 in mouse cells

    Depositor

    Yusuke Hirabayashi

    Article

    Suga et al PLoS Biol. 2023 Aug 31;21(8):e3002246. d

    Insert

    shRNA against mouse OPA1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV Cyclin D2-short

    Plasmid

    #21655

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 (CCND2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV Cyclin D2 long-wt

    Plasmid

    #21646

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Cyclin D2 (CCND2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C1-PRKAA2ΔC

    Plasmid

    #30307

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    AMP-activated Protein Kinase α2 ΔC (Prkaa1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids Ser 527 to Arg 552

    Available Since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pSHS248

    Plasmid

    #87354

    Purpose

    S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S/S867C/N1054C

    Promoter

    T7

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET FLIPE-10u

    Plasmid

    #13538

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207R
    

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    A207R in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. coreR2 (1-387)

    Plasmid

    #115648

    Purpose

    expression of truncated Rag2

    Depositor

    Martin Gellert

    Article

    Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

    Insert

    Rag2 core (1-387) (Rag2 Mouse)
    

    Tags

    8XHis-MBP-PreScission

    Expression

    Mammalian

    Mutation

    truncated Rag2 (aa 1-387)

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRegev_ExonSkip_LandingPad

    Plasmid

    #204186

    Purpose

    Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    Beta globin splicing minigene
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    Truncated CAG

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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