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pHS1993

Plasmid

#239836

Purpose

OMEGAOff-KRK mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

DNMT3A-DNMT3L-OrufIscB-KRK-KRAB

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1 + E137K/E4…

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221-LAP3

Plasmid

#217660

Purpose

For gateway cloning of LAP3

Depositor

Karla Feijs

Article

Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

Insert

LAP3 (LAP3 Human)

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-RFPKDEL

Plasmid

#138660

Purpose

Reporter plasmid expressing the ER-located RFP

Depositor

Bertrand Collet

Article

Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

Insert

RFP-KDEL

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-AGG-SpCas9

Plasmid

#214603

Purpose

encode SpCas9; in vitro transcribed modified RNA vector

Depositor

Yuxuan Guo

Article

Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

Insert

SpCas9

Use

CRISPR and Unspecified

Promoter

T7

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMetYCgate

Plasmid

#105074

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

Cub-PLV

Expression

Yeast

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNXgate33-3HA

Plasmid

#105075

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

CAG_oROS-Gr

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Hygro_RGECO1.2

Plasmid

#164592

Purpose

Stable integration and expression of RGECO1.2 in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

R-GECO1.2

Use

Lentiviral

Expression

Mammalian

Mutation

R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GW1-rogRFP2

Plasmid

#207876

Purpose

Mammalian expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-FUSN

Plasmid

#200967

Purpose

induction of phase-separated condensate mediated by FUSN-IDR

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSN

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

Plasmid

#98927

Purpose

Cre dependent AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

GFP-smFP-myc

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-eDHFR-MCS

Plasmid

#172108

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

Depositor

Shinya Tsukiji

Article

Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

Insert

EGFP-eDHFR-20aa (folA E. coli)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PTP1B human

Plasmid

#102719

Purpose

human PTP1B catalytic domain (residues 1-301) in RP1B vector

Depositor

Wolfgang Peti

Article

Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

Insert

Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pYDS2.0_AT118-L

Plasmid

#222847

Purpose

Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Expression

Yeast

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-Flag-Rheb

Plasmid

#165031

Purpose

expression of Flag tagged Rheb protein in mammalian cells

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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