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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,039 results for: pET

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Showing: 3461 - 3480 of 10039 results

  3. KRas FAK biosensor

    Plasmid
    #78301
    Purpose
    FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells
    Depositor
    Yingxiao Wang
    Publication
    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRas FAK biosensor
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90473-plasmid-map-sequence-id-191118

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  9. pKK-CyOFP1-TEV

    Plasmid
    #105782
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    CyOFP1-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105782-plasmid-map-sequence-id-203371

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  11. hFerritinL

    Plasmid
    #122653
    Purpose
    Expression of hFerritinL in bacterial cells
    Depositor
    Trevor Douglas
    Insert
    light chain ferritin, human (FTL Human)
    Expression
    Bacterial
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122653-plasmid-map-sequence-id-237551

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  14. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Publication
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

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  17. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162685-plasmid-map-sequence-id-320886

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  18. pcDNA3.1-mASS1

    Plasmid
    #34576
    Depositor
    Peter Murray
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ASS1 (Ass1 Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    34576-plasmid-map-sequence-id-201439

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  19. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Publication
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Tags
    E6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 3461 - 3480 of 10039 results