We narrowed to 18,769 results for: puro

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CROP_C9_Puro_BCL2

Plasmid

#183272

Purpose

All-in-One CRISPRko system with a guide RNA that targets BCL2 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BCL2

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AR

Plasmid

#183271

Purpose

All-in-One CRISPRko system with a guide RNA that targets AR gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AR

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ALK

Plasmid

#183270

Purpose

All-in-One CRISPRko system with a guide RNA that targets ALK gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ALK

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ADA

Plasmid

#183269

Purpose

All-in-One CRISPRko system with a guide RNA that targets ADA gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ADA

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_Puro

Plasmid

#167879

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pChd4EosHaloTAF_Puro

Plasmid

#172099

Purpose

Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

Depositor

Brian Hendrich

Insert

chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)

Use

Mouse Targeting

Tags

3xFLAG, Avi, Eos, Halo, and TEV

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_MYNN

Plasmid

#45260

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MYNN (MYNN Human)

Use

Retroviral

Expression

Mammalian

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_hcRED

Plasmid

#45286

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

hcRed

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_LRRC31

Plasmid

#45271

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

LRRC31 (LRRC31 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
PB-PEmax

Plasmid

#187893

Purpose

All-in-one prime editor piggyBac transposon with PEmax

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EML4-ALK variant 1

Plasmid

#183828

Purpose

Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

Depositor

Aaron Hata

Publication

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-13 and ALK exon…

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Publication

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Promoter

EF1

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Human sgRNA library Gattinara in pRDA_118 backbone

Pooled library

#136986

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.

Depositor

John Doench

Publication

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Publication

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Promoter

Doxycycline inducible

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-Cassette mCherry

Plasmid

#102943

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (mCherry version)

Depositor

David Scheinberg

Publication

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MED12_pLX307

Plasmid

#98350

Purpose

Lentiviral expression of MED12

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MED12 (MED12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R1392Q

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV_Zika_NS2B_Flag

Plasmid

#79637

Purpose

Third generation Self-inactivating lentivector expressing NS2B from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS2B

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMRXIP GFP-Stx17TM

Plasmid

#45910

Depositor

Noboru Mizushima

Publication

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

GFP

Mutation

deleted amino acids 1-228

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
MSCV-C 18E7

Plasmid

#37886

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 18 E7 (E7 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles