176,505 results
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Plasmid#145031PurposeExpress SARS-CoV spike protein with C9 tag at C-terminal in mammalian cellsDepositorInsertSARS-CoV Spike (S Synthetic)
TagsC9 and CD5 signal peptideExpressionMammalianPromoterCMVAvailable SinceApril 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro
Plasmid#141379PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG153 FKBP-CFAST11
Plasmid#130813PurposeExpresses FKBP-CFAST11 in mammalian cellsDepositorInsertFKBP-CFAST11
TagsMyc-tagExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (AAV1)
Viral Prep#104493-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (#104493). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7b-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7b expression. jGCaMP7b exhibits the brightest resting fluorescence and can be used for imaging of small neuronal processes (dendrites and axons). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-B19G (AAV1)
Viral Prep#100799-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-TREtight-mTagBFP2-B19G (#100799). In addition to the viral particles, you will also receive purified pAAV-TREtight-mTagBFP2-B19G plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA (Addgene# 100798). These AAV preparations are suitable purity for injection into animals.DepositorPromoterTREtightTagsBFP (in presence of Cre and 100798)Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV Retrograde)
Viral Prep#104492-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterSynAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato
Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
TagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRExpressionBacterialPromoterpRhamBAD and ptetAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-hSyn-mCherry
Plasmid#87916PurposesgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)DepositorInsertmCherry
UseAAV and CRISPRExpressionMammalianPromoterhSynAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
TagsHisExpressionBacterialMutationE54K and L372PAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRa-v2, genome-wide, top 5 sgRNAs/gene
Pooled Library#83978PurposeVersion 2 of the human CRISPRa genome wide libraryDepositorUseLentiviralAvailable SinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human genome-wide lentiviral CRISPR gRNA library version 1
Pooled Library#67989PurposeKnockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.DepositorSpeciesHomo sapiens, Mus musculus, and SyntheticUseCRISPR and LentiviralAvailable SinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XFLAG-EGFP-OMP25
Plasmid#83354PurposeFor tagging mitochondria with Sigma FLAG epitopesDepositorInsert3XFLAG-EGFP-OMP25
UseRetroviralExpressionMammalianAvailable SinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIP-Flag-TfebAA
Plasmid#79014PurposeFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.DepositorAvailable SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GoldenBraid 2.0 Kit
Plasmid Kit#1000000076PurposePlant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector TypePlant ExpressionEditing TypeCRISPRCloning TypeGolden Gate (GoldenBraid)Available SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Tet3
Plasmid#60940PurposeExpresses Tet3 in mammalian cellsDepositorInsertTen-Eleven Translocation 3
TagsflagExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-N1
Plasmid#54594PurposeLocalization: N1 Cloning Vector, Excitation: 516, Emission: 529DepositorTypeEmpty backboneTagsmCitrineExpressionMammalianAvailable SinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLSSmOrange-mKate2 caspase-3 biosensor
Plasmid#37132DepositorInsertLSSmOrange-DEVD-mKate2
ExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only