We narrowed to 12,099 results for: VARS
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Plasmid#191856PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only
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Ppst93-MCRmar-NG
Plasmid#192766PurposeExpresses NG-tagged MCRmar in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-CA
Plasmid#192765PurposeExpresses CA-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NB
Plasmid#192764PurposeExpresses NB-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-MCRaeo-NG
Plasmid#192763PurposeExpresses NG-tagged MCRaeo in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCK411.RR1
Plasmid#192644PurposeExpresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpRDepositorInsertBBa_J23119-RR1
UseCRISPR and Synthetic BiologyPromoterBBa_J23119Available SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR
Plasmid#189943PurposeLentiviral vector to co-express an spsgRNA with NLS-tagBFPDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-MYC-DYK-N72A-N199A
Plasmid#115252PurposeFor mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tagDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-MYC-DYK-C312A
Plasmid#115253PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tagDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-V5-Nxph1
Plasmid#184336PurposeExpression of V5-tagged mouse neurexophilin-1DepositorAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST_LIM1224
Plasmid#182041PurposeFor expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.DepositorAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST_LIM1334
Plasmid#182040PurposeFor expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.DepositorAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML104-natR412
Plasmid#162042PurposeExpresses Cas9 and contains natR412 guide which targets the natR geneDepositorInsertnatR412 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML107-natR854
Plasmid#162043PurposeExpresses Cas9 and contains natR854 guide which targets the natR geneDepositorInsertnatR854 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_10_0460_P460G_D463N_S465A
Plasmid#171645PurposeExpression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460 P460G D463N S465A
TagsMyc-HisExpressionMammalianMutationP460G; D463N; S465AAvailable SinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH191
Plasmid#172149PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
ExpressionBacterialMutationgIII(P29*, Y184*); [strong promoter eG, strong RB…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH190
Plasmid#172148PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
ExpressionBacterialMutationgIII(P29*); [strong promoter eG, strong RBS SD8] …Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyExpressionMammalianPromoterCMV-tetO2Available SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD
Plasmid#168144PurposeInducible expression of AvCAST TnsA, TnsB and TnsC proteins.DepositorInsertAvCAST Tns proteins (TnsA, TnsB and TnsC)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS9: pTns(AvCAST)_ΔTniQ
Plasmid#168142PurposeInducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.DepositorInsertsAvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only