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pXPR_016-sgATF4-1

Plasmid

#202455

Purpose

Expression of a guide RNA targeting ATF4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

ATF4 gRNA (ATF4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pαH-S-HRV3C

Plasmid

#164567

Purpose

SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike - Furin site changed for HRV3C site

Tags

2X Strep-Tag II and 8X His tag

Expression

Mammalian

Mutation

682-685 (furin site) replaced with HRV3C site (LE…

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-RRAR

Plasmid

#164569

Purpose

SARS-CoV-2 Spike protein with Furin site restored (S-RRAR variant)

Depositor

Priyamvada Acharya

Article

Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

Insert

Spike (S-RRAR variant)

Tags

2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

Expression

Mammalian

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBullet-tgn-c

Plasmid

#53072

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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Swachmann-Bodian-Diamond Syndrome Gene, sbds_L (OZ545)

Plasmid

#28070

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds (sbds Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
dopamine beta hydroxylase (dbh)_L (OZ525)

Plasmid

#27200

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting dbh (dbh Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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cftr_R (OZ518)

Plasmid

#27193

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting cftr (cftr Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-BRAT1

Plasmid

#161920

Purpose

Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

BRCA1-associated ATM activator 1 (BRAT1 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-M-3A2

Plasmid

#73435

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor 3A2 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Constitutive wild-type S. pyogenes promoter

Available Since

March 3, 2016

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Dre Flp-in replacement vector

Plasmid

#61571

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Dre (Pvalb D6 Bacteriophage, Mouse)

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON

Plasmid

#180423

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

Depositor

Priyamvada Acharya

Article

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-TARDBP-A315T_VA

Plasmid

#141329

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cells

Depositor

Mohan Babu

Article

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-A315T (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Mutation

A315T

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_POT1_WT

Plasmid

#81820

Purpose

Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POT1 (POT1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_YTHDC1_WT

Plasmid

#81799

Purpose

Gateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

YTHDC1 (YTHDC1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MEF2C_WT

Plasmid

#81735

Purpose

Gateway Donor vector containing MEF2C , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MEF2C (MEF2C Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMARCB1_WT

Plasmid

#81743

Purpose

Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMARCB1 (SMARCB1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R505C

Plasmid

#81507

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

R505C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLD_WT

Plasmid

#82326

Purpose

Gateway Donor vector containing CYLD , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLD (CYLD Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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