We narrowed to 4,454 results for: TIL

1. pTRE-BI-osTIR1

   Plasmid

   #207840

   Purpose

   Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   osTIR
   mCherry
   mAID_-VHH-GFP4
   

   Use

   Tags

   3X HA-Tag and weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV-indLS2

   Plasmid

   #123068

   Purpose

   Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.

   Depositor

   Kevin Brindle

   Article

   Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1

   Inserts

   CreERT2 with intron
   Firefly luciferase
   mStrawberry
   

   Use

   Lentiviral and Luciferase; Fluorescence

   Tags

   Expression

   Mammalian

   Mutation

   synthetic intron added as indicated

   Promoter

   CAGGS (after Cre mediated inversion) and CAGGS (f…

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

   Plasmid

   #201120

   Purpose

   RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

   Depositor

   Ori Bar-Nur

   Article

   Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

   Insert

   TurboRFP; H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MCP-NMS

   Plasmid

   #210705

   Purpose

   This plasmid express EF1a promoter driven MCP-NMS transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-sfCherry11-ß-actin

   Plasmid

   #70221

   Purpose

   Expresses sfCherry11-ß-actin in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   sfCherry11-actin (ACTB Human)
   

   Use

   Tags

   sfCherry11

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mVenus-PA-Rac1

   Plasmid

   #22007

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Use

   Tags

   6X His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

7. LARG mCherry sspB in pcDNA3.1

   Plasmid

   #90459

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mCherry
   sspB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R73Q

   Promoter

   CMV

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-TRE-FH-TET3 short

   Plasmid

   #166916

   Purpose

   Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

   Depositor

   Roland Friedel

   Article

   Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

   Insert

   TET3 short isoform
   

   Use

   Lentiviral

   Tags

   HA Tag

   Expression

   Mammalian

   Mutation

   Changed nucleotide 18 (G) to (A) to delete FseI r…

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

   Plasmid

   #168107

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   Cas9-P2A-mCherry
   

   Use

   CRISPR and Cre/Lox

   Tags

   HA

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

    Plasmid

    #118594

    Purpose

    Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    sgRNA/Puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX:PGRMC1

    Plasmid

    #136062

    Purpose

    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

    Depositor

    Francisco Domínguez Hernández

    Article

    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

    Insert

    Progesterone receptor membrane component 1 (PGRMC1 Human)
    

    Use

    Tags

    GST-PGRMC1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetO-FUW-Ezh2

    Plasmid

    #118587

    Purpose

    Expresses Ezh2 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-CyOFP1-TEV

    Plasmid

    #105782

    Purpose

    Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    CyOFP1-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-mClover3-TEV

    Plasmid

    #105778

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mClover3-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-BI-aaAFB2

    Plasmid

    #207839

    Purpose

    Inducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    atAFB2
    mCherry
    miniIAA7
    VHH-GFP4
    

    Use

    Tags

    3X FLAG-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-mGas8-PA

    Plasmid

    #176468

    Purpose

    Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    growth arrest specific 8 (Gas8 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRecLV105

    Plasmid

    #107238

    Purpose

    A lentiviral vector for expression in mammalian cells

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p323.hSyn.Halo-mFMRP.Wpre

    Plasmid

    #185909

    Purpose

    Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging

    Depositor

    Young J Yoon

    Article

    Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6

    Insert

    HaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    hSyn (human Synapsin promoter)

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-VTKS4

    Plasmid

    #170855

    Purpose

    AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMH-SFB-USP7

    Plasmid

    #99393

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    USP7 (USP7 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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