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pUC18-mini-Tn7T-Plac-dCas9 (Plac)

Plasmid

#105235

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Fra-2

Plasmid

#187907

Purpose

Expresses Fra-2 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra2 (Fosl2 Mouse)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (TBE 1/2 - wt)

Plasmid

#16595

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (TBE 1/2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
p3XFlag-CMV10-Flag-mMcl-1

Plasmid

#32978

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Tags

3XFlag

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

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pMON-HsNMT1

Plasmid

#67475

Purpose

Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-GCaMP6s-WPRE-sv40

Plasmid

#184414

Purpose

AAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoter

Depositor

Marylka Yoe Uusisaari

Article

Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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BB2_AB

Plasmid

#98534

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-mArg1 base promoter -31/-2365

Plasmid

#34570

Depositor

Peter Murray

Article

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Insert

Arg1 base promoter -31/-2365 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-Rheb

Plasmid

#133767

Purpose

Expresses GFP-tagged Rheb in mammalian cells

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb (RHEB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad5-3'UTR

Plasmid

#61777

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-Stat5a1*6

Plasmid

#130668

Purpose

Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJL33

Plasmid

#227430

Purpose

Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

Depositor

Pierre Santucci

Article

Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

Insert

mWasabi

Use

CRISPR

Expression

Bacterial

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJL34

Plasmid

#227431

Purpose

Kanamycin resistant L5-integrative vector for CRISPR interference in M.tuberculosis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

Depositor

Pierre Santucci

Article

Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

Insert

tdTomato

Use

CRISPR

Expression

Bacterial

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

Plasmid

#105234

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEBG-hUBP43

Plasmid

#12455

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

UBP43 (USP18 Human)

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Zeb2-3'UTR

Plasmid

#61771

Purpose

Luciferase reporter containing the 3'UTR of mouse Zeb2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zeb2 3'UTR

Use

Luciferase

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only