We narrowed to 6,241 results for: nls

1. HaloTag-LacI-KRAB

   Plasmid

   #232638

   Purpose

   lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressor

   Depositor

   Karsten Rippe

   Insert

   HaloTag-LacI-KRAB
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNeoG2

   Plasmid

   #200222

   Purpose

   NeoR nls-mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNeoR2

   Plasmid

   #200223

   Purpose

   NeoR nls-scarlet marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Akaluc VLP

   Plasmid

   #182212

   Purpose

   YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).

   Depositor

   Claudia Vickers

   Inserts

   VP2C-Akaluc
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology; Tem imaging

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-MP-84-spCas9-U6-gRNA

   Plasmid

   #206935

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   MP-84

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHygR2

   Plasmid

   #200071

   Purpose

   hygR selection nls-mScarlet marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1 hPHGDH

   Plasmid

   #154916

   Purpose

   Doxycyclin inducible expression of human PHGDH

   Depositor

   Richard Possemato

   Article

   Richard et al Richard Possemato Lab plasmids

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTagRFP-T2-PCNA-19

   Plasmid

   #58043

   Purpose

   Localization: Nucleus, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   PCNA (PCNA Human)
   

   Tags

   mTagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHygR2r

    Plasmid

    #200072

    Purpose

    hygR selection nls-mScarlet marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHygG2r

    Plasmid

    #200069

    Purpose

    hygR selection nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-tdTomato-VPR

    Plasmid

    #232632

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer

    Depositor

    Karsten Rippe

    Insert

    dCas9-tdTomato-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHygR8

    Plasmid

    #200221

    Purpose

    hygR selection nls-mScarlet marked rapid RMCE integration vector without loxP site

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHygG8

    Plasmid

    #200220

    Purpose

    hygR selection nls-mNG marked rapid RMCE integration vector without loxP site

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKG194

    Plasmid

    #69757

    Purpose

    Mammalian Expression of CENP-T aa 1-375

    Depositor

    Iain Cheeseman

    Insert

    CENP-T aa 1-375 (CENPT Human)
    

    Use

    Retroviral

    Tags

    LacI-NLS and eGFP

    Expression

    Mammalian

    Mutation

    e.coli optimized sequence

    Available Since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. PX398 Nitratifractor salsuginis str DSM 16511 Cas9

    Plasmid

    #68334

    Purpose

    Nitratifractor salsuginis str DSM 16511 Cas9

    Depositor

    Feng Zhang

    Insert

    NsCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBW121-SS-278

    Plasmid

    #68766

    Purpose

    CIS-V2 with N-FLAG and C-HIS

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    CIS-V2
    

    Tags

    HIS, NLS, and flag

    Expression

    Mammalian

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBW121-SS-276

    Plasmid

    #68764

    Purpose

    CIS-V1 with N-FLAG and C-HIS

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    CIS-V1
    

    Tags

    HIS, NLS, and flag

    Expression

    Mammalian

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW121-SS-277

    Plasmid

    #68765

    Purpose

    CIS-V1m with N-FLAG and C-HIS

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Insert

    CIS-V1m
    

    Tags

    HIS, NLS, and flag

    Expression

    Mammalian

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid

    #246982

    Purpose

    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.

    Depositor

    Amro Hamdoun

    Insert

    φC31(PhiC31)
    

    Use

    Synthetic Biology

    Tags

    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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