We narrowed to 8,347 results for: construct

1. CMV-2xFLAG-Atoh1

   Plasmid

   #101734

   Purpose

   Expresses mouse Atoh1 with FLAG tag

   Depositor

   Albert Edge

   Article

   Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046747

   Plasmid

   #149249

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4046733

   Plasmid

   #145665

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTRE:shRNA A-pGK:rtTA-Cre

   Plasmid

   #68471

   Purpose

   Inducible lentivirus expressing an arbitrary shRNA and Cre

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   GFP-shRNA A-miR30
   rtTA-2A-Cre
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pGK and pTRE

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUS_A3B

   Plasmid

   #43852

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFUS_A4A

   Plasmid

   #43853

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4136464

   Plasmid

   #149534

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pU1/Sm/3' Box_(GLuc)_TOP1

   Plasmid

   #68437

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U1

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV Puro-UBC:GFP

   Plasmid

   #68482

   Purpose

   Retrovirus expressing GFP driven by UBC promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and UBC

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4136461

    Plasmid

    #149538

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pGBW-m4046758

    Plasmid

    #145670

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCMV/3' Box_(GLuc)_TOP2

    Plasmid

    #68435

    Purpose

    Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP2 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFGC5941-PacI-ath-STTM2937

    Plasmid

    #84112

    Purpose

    target miRNA2937 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM2937
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV Puro-SV40:GFP

    Plasmid

    #68483

    Purpose

    Retrovirus expressing GFP driven by SV40 promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and SV40

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFGC5941-PacI-ath-STTM159

    Plasmid

    #84084

    Purpose

    target miRNA159 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM159
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFGC5941DR5-PacI-ath-STTM390

    Plasmid

    #84117

    Purpose

    target miRNA390 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM390
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-FKBP-HA no-pA

    Plasmid

    #82598

    Purpose

    HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FKBP-HA (no pA)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFGC5941-PacI-ath-STTM858a/b

    Plasmid

    #84177

    Purpose

    target miRNA858 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM858a/b
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4046789

    Plasmid

    #145660

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. MSCV Puro-EFS:GFP

    Plasmid

    #68484

    Purpose

    Retrovirus expressing GFP driven by EFS promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge