We narrowed to 22,781 results for: Sis

1. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-CRY2-mCherry-MYPT169

   Plasmid

   #178526

   Purpose

   Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CRY2-mCherry-MYPT169
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC-CRISPR-P2A-BlastR

   Plasmid

   #98147

   Purpose

   Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   P2A-BlastR fusion with Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ad-Flag-XBP1u

   Plasmid

   #63677

   Purpose

   Adenoviral vector for XBP1u expression

   Depositor

   Lingfang Zeng, Qingbo Xu

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   unspliced

   Promoter

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

5. L309-myc-seipin-WT

   Plasmid

   #64061

   Purpose

   myc-tagged murine seipin (wild type); lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

   Insert

   bscl2 (Bscl2 Mouse)
   

   Use

   Lentiviral

   Tags

   myc epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available Since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

   Plasmid

   #127314

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOP/SSAT

   Plasmid

   #140291

   Purpose

   Expresses human SSAT in bacterial cells

   Depositor

   Gaetano Marverti, Saverio Bettuzzi

   Article

   Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

   Insert

   SSAT1 (SAT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_eA3A-nCas9

   Plasmid

   #163535

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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