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We narrowed to 31,681 results for: PLE

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Showing: 3541 - 3560 of 31681 results

  5. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199368
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    3C-SUMOstar-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199368-plasmid-map-sequence-id-389900

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  10. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  11. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  17. pCIG1_UL136_19kDa_EE

    Plasmid
    #74951
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    Glu-Glu tag (EYMPME)
    Expression
    Mammalian
    Mutation
    delta 1-127
    Promoter
    CMV, lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74951-plasmid-map-sequence-id-218061

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  18. pCIG1_UL136_23kDa_Myc

    Plasmid
    #74949
    Purpose
    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.
    Depositor
    Felicia Goodrum
    Article
    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:
    Insert
    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    Use
    Lentiviral
    Tags
    HA Tag (YPYDVPDYA)
    Expression
    Mammalian
    Mutation
    delta 1-99 M128A
    Promoter
    CMV, Lentiviral LTR
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74949-plasmid-map-sequence-id-218062

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Showing: 3541 - 3560 of 31681 results