We narrowed to 23,834 results for: Spr;

1. pCfB3051(gRNA X-3 XI-2 XII-2)

   Plasmid

   #73293

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHS1068

   Plasmid

   #205962

   Purpose

   NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH proteins and CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac (proteins), pJ23119 (crRNA)

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCSN061

   Plasmid

   #101725

   Purpose

   Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PLK1 G5.1 gRNA

   Plasmid

   #90834

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PLK1 (Guide Designation G5.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBBK83 pHomL-RPL18Bp-ERG6-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179067

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Scarlet(rfp), a marker for the lipid droplet

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-ERG6-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBBK82 pHomL-RPL18Bp-Scarlet(rfp)-Link-ATG8-SSA1t-HomR (AmpR)

   Plasmid

   #179066

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-ATG8, a marker for the autophagosome

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-ATG8
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pY005 (pcDNA3.1-hLb3Cpf1)

   Plasmid

   #69977

   Purpose

   Expresses humanized Lb3Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb3Cpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCMV_EE-nCas9

   Plasmid

   #163541

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   EE-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBBK76 pHomL-RPL18Bp-PIL1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179060

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Scarlet(rfp), a marker for the eisosomes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-PIL1-Link-Scarlet(rfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109311

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Insert

    Ldlr gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-sgGAL4-4

    Plasmid

    #46916

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgGAL4-4
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

    Plasmid

    #179054

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-TUB1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIK208

    Plasmid

    #65630

    Purpose

    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KIF2A D7.2 gRNA

    Plasmid

    #90720

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF2A (Guide Designation D7.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KIF2A D8.2 gRNA

    Plasmid

    #90721

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF2A (Guide Designation D8.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PSMD14 E6.4 gRNA

    Plasmid

    #90859

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PSMD14

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PSMD14 (Guide Designation E6.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTox-EGFPsite1_NGG-PAM (BPK740)

    Plasmid

    #181746

    Purpose

    p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)
    

    Use

    Selection plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATAD2 D7.3 gRNA

    Plasmid

    #90538

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATAD2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ATAD2 (Guide Designation D7.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ-dAaCas12b-Act3.0

    Plasmid

    #158413

    Purpose

    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge