We narrowed to 32,188 results for: uros

1. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

   Plasmid

   #83107

   Purpose

   3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

   Depositor

   Andrew Brooks

   Inserts

   c-Myc (MYC Human)
   CDK4 (CDK4 Human)
   eGFP
   

   Use

   Lentiviral

   Tags

   2A self cleaving peptide

   Expression

   Mammalian

   Mutation

   R24C and p64 isoform

   Promoter

   hPGK and hPGK (single transcript)

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

   Plasmid

   #137994

   Purpose

   Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

   Depositor

   Amy Brock

   Article

   Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR

   Plasmid

   #171596

   Purpose

   Ectodomain deleted negative control ACE2 from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   ACE2 (ACE2 Human)
   mCherry
   H2A
   Pac
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP

   Plasmid

   #211342

   Purpose

   Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

   Depositor

   Monkol Lek

   Insert

   m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73032

   Purpose

   Express fluorescent and luminescence dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Truncated EF1

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

   Plasmid

   #181973

   Purpose

   Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-BBz CAR; LTBR (LTBR Synthetic, Human)
   

   Use

   Lentiviral

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-puro FLAG wildtype, catalytic active lipin 1

   Plasmid

   #32010

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   lipin1 (Lpin1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

   Plasmid

   #181972

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; LTBR (LTBR Synthetic, Human)
   

   Use

   Lentiviral

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #211344

    Purpose

    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

    Depositor

    Monkol Lek

    Insert

    m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
    

    Use

    TALEN

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 1071 pBabe puroL myr Akt T308A S473A

    Plasmid

    #9014

    Depositor

    William Sellers

    Article

    DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

    Insert

    Akt1 T308A S473A (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    T308A S473A

    Available Since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

12. RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)

    Plasmid

    #139447

    Purpose

    Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1053 pBabe puroL Akt K179M T308A S473A

    Plasmid

    #9013

    Depositor

    William Sellers

    Article

    DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

    Insert

    Akt1 K179M T308A S473A (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K179M T308A S473A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro

    Plasmid

    #173228

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-MTS-ccdb-DddA(G1333N)-UGI-Puro

    Plasmid

    #173229

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164793

    Purpose

    Express miR-144~451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid

    #209086

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

    Plasmid

    #170467

    Purpose

    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Bakhache et al bioRxiv 2021

    Insert

    Nucleocapsid
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

    Plasmid

    #210605

    Purpose

    heat shock promoter - activated hyperdCas12a-based transcriptional base editor

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    ABE8e-hyperdCas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    truncated hsp70

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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