We narrowed to 62,257 results for: cloning
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Plasmid#154877PurposeExpresses mGSDME in mammalian cellsDepositorAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only
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pAtetO_6xHis-Bst-LF
Plasmid#145799PurposePlasmid for the aTc-inducible expression of the large-fragment of Bst DNAPDepositorInsertBst-LF
Tags6xHisExpressionBacterialPromotertet PAAvailable SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-X1-Neo-HA-UFL1
Plasmid#139860PurposeLentiviral expression of HA-tagged human UFL1DepositorAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-mCh
Plasmid#120169PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)DepositorInsertkinesin family member C1 (KIFC1 Human)
TagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mC-G-LC3B-P
Plasmid#124974Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseLentiviralPromoterCMVAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
TagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialPromoterpDABAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhU6 and hUbCAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-AK1-His
Plasmid#118977PurposeBacterial expression plasmid for Adenylate kinase isoenzyme 1DepositorInsertadenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
Tags6x-HisExpressionBacterialPromoterT7 promoterAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-mCherry-lenti
Plasmid#115796PurposeExpresses mCherry in human cells, contained in a lentiviral vector.DepositorInsertmCherry
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1382
Plasmid#111436PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.657
Plasmid#108525PurposeCymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.DepositorInsertCymRAM + PCymRC-YFP
ExpressionBacterialMutationCymR: S110G A171VAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-nEF-dCas9
Plasmid#106430PurposeExpresses SpdCas9 from the nEF promoter in AAV backboneDepositorInsertnEF-dCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianPromoternEFAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-V5-KDM3A
Plasmid#82331Purposelentiviral expression of KDM3ADepositorInsertKDM3A (KDM3A Human)
UseLentiviralTagsV5ExpressionMammalianMutationtwo silent mutations to make it siRNA resistantAvailable SinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-sfGFP
Plasmid#71739PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertsfGFP
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGK-destination
Plasmid#60436PurposeDestination vector with PGK - Hygromycin selection markerDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMZ379
Plasmid#74215PurposeCombined sgRNA/Cas9 vector with NatMX markerDepositorInsertsCas9
rrk1:sgRNA
UseCRISPRExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available SinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTKW106alp7A
Plasmid#69360PurposeStabilized lacZ expression vector containing alp7A and hok/sokDepositorInsertslacZ
alp7A
hok
placIq-lacI
ExpressionBacterialPromoterpTac and placIqAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-2a-tdTomato
Plasmid#67707Purpose3' entry 2A-tdTomatoDepositorInsert2A-tdTomato
MutationG172S (please see depositor's comments below)Available SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
Plasmid#50022Purposeexpresses Membrane tagged G-CaMP3 in cells expressing CreDepositorInsertG-CaMP3
UseAAVTagsMARCKS sequence (MGCCFSKT)PromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only