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We narrowed to 8,836 results for: construct

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Showing: 3561 - 3580 of 8836 results

  1. pGBW-m4046761

    Plasmid
    #145582
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp11
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145582-plasmid-map-sequence-id-287759

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  2. pGBW-m4046777

    Plasmid
    #145583
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145583-plasmid-map-sequence-id-287760

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  3. pGBW-m4046734

    Plasmid
    #145576
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145576-plasmid-map-sequence-id-287753

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  4. pGBW-m4046826

    Plasmid
    #145687
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145687-plasmid-map-sequence-id-292489

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  9. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

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  10. pTH846-gst-dissc

    Plasmid
    #96907
    Purpose
    Plasmid for yeast expression of GST encoded by non-preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon dis-optimised glutathione-S transferase
    Tags
    8xhistidine
    Expression
    Yeast
    Promoter
    TDH3
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96907-plasmid-map-sequence-id-206054

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  11. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

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  12. pTH858-gst-optec V156E

    Plasmid
    #89592
    Purpose
    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed valine 156 to glutamic acid
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89592-plasmid-map-sequence-id-206041

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  13. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

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  15. pRPa-c6MYCn

    Plasmid
    #69242
    Purpose
    Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Promoter
    RRNA promoter
    Available Since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69242-plasmid-map-sequence-id-123680

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  16. pRP-c6MYCn

    Plasmid
    #69243
    Purpose
    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Article
    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Tags
    cMyc
    Promoter
    RRNA promoter
    Available Since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69243-plasmid-map-sequence-id-123691

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  17. pHJ33

    Plasmid
    #48362
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48362-plasmid-map-sequence-id-70053

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Showing: 3561 - 3580 of 8836 results