We narrowed to 10,167 results for: otos

1. pET-BoNT/X

   Plasmid

   #168017

   Purpose

   Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   BoNT/X-LC(1-439)-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. KillerOrange /pQE-30

   Plasmid

   #74748

   Purpose

   KillerOrange gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al PLoS One. 2015 Dec 17;10(12):e0145287. d

   Insert

   KillerOrange
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   KillerRed with substitutions G3C Y66W D113S N145S…

   Promoter

   T5

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

   Plasmid

   #198450

   Purpose

   Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-His6-GFP-C-LACE

   Plasmid

   #133913

   Purpose

   Bacterial expression of His6-GFP-C-LACE

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-AlkB deltaN11

   Plasmid

   #73622

   Purpose

   Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

   Depositor

   Catherine Drennan

   Article

   Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   AlkB (alkB E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deletion of first 11 AA

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

6. sAB-MBP

   Plasmid

   #204736

   Purpose

   synthetic antigen-binding fragment specifically recognizing bacteria maltose-binding protein

   Depositor

   Minglei Zhao

   Article

   Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

   Insert

   synthetic antigen-binding fragment recognizing bacteria maltose-binding protein
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-CAG-Brainbow3.2-PGK-PuroTK

   Plasmid

   #159928

   Purpose

   Brainbow transgenesis

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

   Inserts

   NFPnls
   mOrange2
   EGFP
   mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ER-EGFP-eMagA

   Plasmid

   #162245

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   CytP450 ER targeting sequencel-EGFP-eMagA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRXIP-3xMyc-STX17FL

   Plasmid

   #89942

   Purpose

   Expresses syntaxin17 tagged with Myc in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

   Insert

   human syntaxin17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSb_init containing the MBP sybody Sb_MBP#1

    Plasmid

    #132699

    Purpose

    expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    sybody_MBP#1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKII-GRAB_g5-HT3.0

    Plasmid

    #208723

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMD.RVG.CVS24-B2c

    Plasmid

    #19713

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-B2c glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. APneg-VAMP1a

    Plasmid

    #168007

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-Rab5

    Plasmid

    #31733

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab5 (Rab5 Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPCK1

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHYX138

    Plasmid

    #202815

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScADH2

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBR322-Pdp1_NTD-Cre-HiBiT

    Plasmid

    #198318

    Purpose

    Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-Cre-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tph-1(short)::rpl-22-HA

    Plasmid

    #122203

    Purpose

    C. elegans tagged ribosome expression plasmid

    Depositor

    Steven Flavell

    Article

    Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

    Insert

    tph-1(short)::rpl-22-HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-RSET-iGABASnFR2-6xHis

    Plasmid

    #218885

    Purpose

    Bacterial expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    T7

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX208_CMV::mCherry-myc-VAMP2

    Plasmid

    #199716

    Purpose

    Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-myc-VAMP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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