-
Plasmid#27461DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationGlutamine 331 mutated to Lysine (Q331K)PromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMA3379
Plasmid#46874PurposeA lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3DepositorInsertsoluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
UseLentiviralTagsMycExpressionMutationPromoterTRE-TightAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos4b-V69T
Plasmid#98572Purposephotoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertmEos4b-V69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKM-IRES-hrGFP
Plasmid#73405PurposeMammalian expression of mouse PKM and hrGFPDepositorInsertPKM (Prkcz Mouse)
UseTags3xFLAG and pIRES-hrGFP-2aExpressionMammalianMutationtruncated, constitutively active formPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa2-A69T
Plasmid#98578Purposephotoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertpcDronpa2-A69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mKikGR-V69T
Plasmid#98574Purposephotoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmKikGR-V69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA3383
Plasmid#46875PurposeA retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3DepositorInsertsoluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceAug. 13, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
FusionRed-Sit-N-15
Plasmid#56133PurposeLocalization: Golgi TGN, Excitation: 580, Emission: 608DepositorInsertSit (ST6GAL1 Human)
UseTagsFusionRedExpressionMammalianMutationaa 1-45 of SiTPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXW117As(RS-E11)
Plasmid#123145PurposeArsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ117, ParsR, PhrpLE, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 G294A
Plasmid#27459DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
beta4-nAChR shRNA (Beta-4.3)
Plasmid#86650Purposeexpression of shRNA targeting CHRNB4DepositorInsertCHRNB4 (beta4-nAChR) (CHRNB4 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGK-HA-PGRN
Plasmid#234872PurposeLentiviral plasmid expressing HA-tagged human progranulin.DepositorInsertProgranulin (GRN Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHC027 (∆pemK)
Plasmid#226204PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.DepositorInsertUpstream/downstream homology arms for addiction system toxinPemK.
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
LT3GEPIR-WT1shRNA
Plasmid#220560PurposeTo inducibly knockdown WT1 or EWSR1::WT1 expressionDepositorInsertWT1 shRNA (WT1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWalium Htt19Q-mCherry
Plasmid#201248Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertHuntingtin (HTT Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST ɑSynWT-EGFP
Plasmid#201249Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertɑ-Synuclein (SNCA Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::tse5-CT
Plasmid#192958PurposeFor expression of Tse5-CT (1169-1317) in P. putida for biological function studiesDepositorInserttse5-CT
UseTagsExpressionBacterialMutationencodes for tse5 (1169-1317)PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla–insM
Plasmid#191226PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved methionine at 122 in the protein sequencePromoterPH and p10Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chinchilla+N122D–insM
Plasmid#191227PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved methionine at 122 and replaced asparagine…PromoterPH and p10Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse–insD
Plasmid#191228PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 in the protein seque…PromoterPH and p10Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2-IR2 GFP pZa
Plasmid#170083PurposeGFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2-IR2Available sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMA3411
Plasmid#46877PurposeA retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3 double mutant (E473K and C541D)DepositorInsertsoluble guanylate cyclase1 beta 3 (Gucy1b3 Rat)
UseRetroviralTagsExpressionMutationE473K, C541DPromoterLTRAvailable sinceAug. 14, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
PUC57_BAG3wt-eGFP
Plasmid#98182PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorInsertBAG3wt-eGFP (BAG3 Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagExpressionMutationPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-CRBN-P2A-Hygro
Plasmid#124303PurposeLentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoterDepositorInsertCRBN (CRBN Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2/103Q
Plasmid#1385DepositorInserthuntingtin (103Q) (HTT Human)
UseTagsEGFP and FLAGExpressionYeastMutationExon1 sequences containing the first 17 amino aci…PromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
GST-TEV-FUS
Plasmid#29629DepositorInsertFUS (FUS Human)
UseTagsGST and TEVExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-T17N
Plasmid#12976DepositorInsertCdc42 DN (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterCMVAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Cre
Plasmid#98039PurposeSecond-generation rabies viral vector genomeDepositorInsertmCre
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Flpo
Plasmid#98040PurposeSecond-generation rabies viral vector genomeDepositorInsertFlpo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E_BAG3wt-eGFP
Plasmid#98180PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorInsertBAG3wt-eGFP (BAG3 Human)
UseGateway vectorTagsGFPExpressionMutationPromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_MAPT:EGFP
Plasmid#92196PurposeLentiviral overexpression vector to make stable bicistronic cell line for Tau screenDepositorInsertDsRed-IRES-MAPT-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-eEF2K
Plasmid#110160PurposeExpression of human eEF2K in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_SNCA:EGFP
Plasmid#92195PurposeLentiviral overexpression vector to make stable bicistronic cell line for α-Syn screenDepositorInsertDsRed-IRES-SNCA-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
UseTagsExpressionBacterialMutationPromoterPosmY, PsulA, and grpEAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
VN-alpha Synuclein
Plasmid#89470Purposeexpresses human alpha Synuclein fused to the N-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
alpha Synuclein-VC
Plasmid#89471Purposeexpresses human alpha Synuclein fused to the C-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsC-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1469
Plasmid#142800PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTOX3 (TOX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11-RinA)2
Plasmid#123146PurposeArsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ117, ParsR, PhrpLE, Pecf11Available sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBItetDT960GFP
Plasmid#80419Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FusionRed-Fascin-C-10
Plasmid#56117PurposeLocalization: Cytoskeleton, Excitation: 580, Emission: 608DepositorInsertFascin (FSCN1 Human)
UseTagsFusionRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF1472
Plasmid#141825PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTOX2 (TOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLC-HA active Nedd8-P2A-Hygro
Plasmid#124307PurposeLentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoterDepositorInsertNedd8 (NEDD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationFive C-terminal amino acids missing compared to w…PromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBItetDT480GFP
Plasmid#96905Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15DepositorInsertEGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalTagsExpressionMammalianMutationPromotertetracycline responsive and bidirectionalAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBItetDMPKSGFP
Plasmid#96903Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalTagsExpressionMammalianMutationPromotertetracycline responsive and bidirectionalAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLC-CUL4A WT-P2A-Puro
Plasmid#124304PurposeLentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoterDepositorInsertCUL4A (CUL4A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA-E11)2
Plasmid#123151PurposeMercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only