We narrowed to 1,068 results for: golden gate assembly
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Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA55
Plasmid#174768PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E without STOP codon
UseUnspecifiedMutationL17EAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA56
Plasmid#174769PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E/D481V without STOP codon
UseUnspecifiedMutationL17E/D481VAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA54
Plasmid#174767PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-D481V without STOP codon
UseUnspecifiedMutationD481VAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA53
Plasmid#174766PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn without STOP codon
UseUnspecifiedMutationWTAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko1_FRTcamFRT
Plasmid#243900PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertcat
ExpressionBacterialAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko1_FRTtetFRT
Plasmid#243901PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInserttetA
ExpressionBacterialAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko2_FRTspcFRT
Plasmid#243907PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertaadA
ExpressionBacterialAvailable SinceJune 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-AtUBQ10
Plasmid#250923PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by AtUBQ10 promoterDepositorInsertAtUBQ10
UseCRISPRExpressionPlantAvailable SinceApril 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-AtUBQ10-pT
Plasmid#250924PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by AtUBQ10 promoterDepositorInsertAtUBQ10
UseCRISPRExpressionPlantAvailable SinceApril 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143-AtUBQ10
Plasmid#250985PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by AtUBQ10 promoterDepositorInsertAtUBQ10
UseCRISPRExpressionPlantAvailable SinceApril 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM352
Plasmid#248764Purpose160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate AssemblyDepositorInsertT7Te-rrnBT1-160xTetO
ExpressionBacterialAvailable SinceMarch 25, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko2_FRTkanFRT
Plasmid#243905PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertaph
ExpressionBacterialAvailable SinceFeb. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko2_FRTcamFRT
Plasmid#243904PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertcat
ExpressionBacterialAvailable SinceFeb. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko2_FRTtetFRT
Plasmid#243903PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInserttetA
ExpressionBacterialAvailable SinceFeb. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko2_FRTampFRT
Plasmid#243906PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertbla
ExpressionBacterialAvailable SinceFeb. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko1_FRTampFRT
Plasmid#243898PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertbla
ExpressionBacterialAvailable SinceJan. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko1_FRTkanFRT
Plasmid#243899PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertaph
ExpressionBacterialAvailable SinceJan. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pR6Ko1_FRTspcFRT
Plasmid#243902PurposeGoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.DepositorInsertaadA
ExpressionBacterialAvailable SinceJan. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM413
Plasmid#248765Purpose10xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate AssemblyDepositorInsertT7Te-rrnBT1-10xTetO
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEM415
Plasmid#248767Purpose40xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate AssemblyDepositorInsertT7Te-rrnBT1-40xTetO
ExpressionBacterialAvailable SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBTK1058
Plasmid#209548PurposeBTK Type 1 homology arm for Golden Gate assemblyDepositorInsertUpstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW622
Plasmid#229868PurposeEntry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)DepositorInsertreverse FRT3 (rFRT3)
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK1059
Plasmid#209549PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertDownstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyAvailable SinceFeb. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZA52
Plasmid#174765PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E/D481V with STOP codon
UseUnspecifiedMutationL17E/D481V + STOP codonAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA50
Plasmid#174763PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-D481V with STOP codon
UseUnspecifiedMutationD481V + STOP codonAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA51
Plasmid#174764PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E with STOP codon
UseUnspecifiedMutationL17E + STOP codonAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA49
Plasmid#174762PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_synthesized NbZAR1 (NbZAR1syn) with STOP codon
UseUnspecifiedMutationWT + STOP codonAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0244
Plasmid#162282PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0024
Plasmid#162281PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLY133
Plasmid#130961PurposeEngineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB4
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY131
Plasmid#130956PurposeEngineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB2
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY132
Plasmid#130960PurposeEngineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB3
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY130
Plasmid#130952PurposeEngineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB1
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143
Plasmid#69295PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
p1300-dCas9-HV5
Plasmid#239479PurposeAn intermediate vector is used to assemble three gRNAs via Golden Gate cloning.DepositorInsertUBQ-dCAS9
UseCRISPRExpressionPlantAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1334_pHarbor-PiggyBac_backbone
Plasmid#239706PurposePaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated intDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBTK151
Plasmid#183127PurposeBTK Type 4 reverse promoter part for golden gate assemblyDepositorInsertReverse CP25 promoter and forward rpoC terminator for dsRNA expression
UseSynthetic BiologyAvailable SinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK150
Plasmid#183126PurposeBTK Type 2 promoter part for golden gate assemblyDepositorInsertReverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
UseSynthetic BiologyAvailable SinceMay 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneExpressionBacterial and InsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNM3746
Plasmid#154109PurposeloxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::His-58::FRT3
mex-5p::FLP D5::sl2::mNG::glh2 3'
UseCre/Lox; RmceExpressionWormPromoternot applicableAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-BsaI-EGFP
Plasmid#123136PurposeGolden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertEGFP, mRFP
UseSynthetic BiologyExpressionMammalianPromotercBAAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A8
Plasmid#99882PurposeRecipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.DepositorTypeEmpty backboneUseTALENAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKG0893_pHarbor-LentiX1_backbone
Plasmid#239704PurposePaqCI Golden Gate assembly plasmid encoding the LentiX1 backboneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKG1179_pPV1-EFS
Plasmid#239690PurposeBsaI Golden Gate assembly plasmid encoding the EFS promoterDepositorInsertEFS promoter
UseSynthetic BiologyAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0586_pPV2-mRuby2
Plasmid#239695PurposeBsaI Golden Gate assembly plasmid encoding the mRuby2 CDSDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2039_pPV1-CAG
Plasmid#239686PurposeBsaI Golden Gate assembly plasmid encoding the CAG promoterDepositorInsertCAG promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only