We narrowed to 5,607 results for: Braf;

1. PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

   Plasmid

   #92402

   Purpose

   Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

   Depositor

   Periklis Pantazis

   Article

   Kalyviotis et al (2020) Behavioral and Neural Genetics of

   Inserts

   Dendra2
   Cerulean
   

   Use

   Zebrafish expression

   Tags

   H2B tag and No tag, cytosolic expression

   Expression

   Mutation

   Promoter

   bactin2

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-TgKI-MCS-tdTomato-polyA

   Plasmid

   #174022

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   tdTomato
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-TgKI-MCS-mLanYFP-polyA

   Plasmid

   #174021

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   mLanYFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2-Gal4ff.zf1

   Plasmid

   #61392

   Purpose

   mRNA synthesis of Gal4ff codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Gal4ff.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5'Entry-lck-7.4kb

   Plasmid

   #58891

   Purpose

   provides zebrafish lck promoter (7.4 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 7.4 kb
   

   Use

   Gateway 5' entry clone

   Tags

   Expression

   Mutation

   mutated single nucleotide (T-->C, 1874th nucle…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestattB (pCM268)

   Plasmid

   #68313

   Purpose

   Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

   Depositor

   Christian Mosimann, Leonard Zon

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-krt5

   Plasmid

   #82585

   Purpose

   5' entry vector containing the zebrafish krt5 promoter for epidermis-specific expression

   Depositor

   Kryn Stankunas

   Article

   Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

   Insert

   zebrafish krt5 promoter (krt5 Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-TgKI-MCS-eGFP-MCS-polyA

   Plasmid

   #174024

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

   Plasmid

   #174030

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-Venus-PEST
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

    Plasmid

    #174028

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC19-TgKI-MCS-p2A-tdTomato-polyA

    Plasmid

    #174027

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-tdTomato
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Y40N

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5'Entry-lck-5.5kb

    Plasmid

    #58890

    Purpose

    provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

    Depositor

    Jeffrey Yoder

    Insert

    zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
    

    Use

    Gateway 5' entry clone

    Tags

    Expression

    Mutation

    mutated single nucleotide (T-->C, 13th nucleot…

    Promoter

    lymphocyte-specific protein tyrosine kinase (lck)…

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2-TagRFPT.zf1

    Plasmid

    #61390

    Purpose

    mRNA synthesis of TagRFPT codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    TagRFPT.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-TgKI-MCS-mScarlet-MCS-polyA

    Plasmid

    #174023

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    mScarlet
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

    Plasmid

    #174029

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    p2A-mScarlet
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MLM3639

    Plasmid

    #42252

    Purpose

    Cas9 expression vector

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    Streptococcus pyogenes Cas9-3X Flag
    

    Use

    CRISPR; Zebrafish expression

    Tags

    3X Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MiniCoopR U6:gRNA, mitfa:Cas9

    Plasmid

    #118840

    Purpose

    Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2_8xCTS-ECFP

    Plasmid

    #200249

    Purpose

    Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    8xCTS-ECFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-Cer.zf1

    Plasmid

    #61389

    Purpose

    mRNA synthesis of cerulean codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Cer.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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