We narrowed to 1,428 results for: RCA

1. pDONR-U6gRNA-PGKpuro2APDGFB

   Plasmid

   #136409

   Purpose

   Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BbsI restriction site in the PDGFB sequence (Glu1…

   Promoter

   U6, PDK

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR-U6gRNA-PGKpuro2ABFP

   Plasmid

   #136405

   Purpose

   Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BbsI restriction site at position 437 was removed…

   Promoter

   U6, PDK

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV-gRNA-Bcan-Ntrk1_4

   Plasmid

   #136413

   Purpose

   Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV-gRNA-Myb-Qk_1

   Plasmid

   #136415

   Purpose

   Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Myb)_U6_sgRNA(Qk)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. JI401: Ad5 E1A/Hexon qRT-PCR standard

   Plasmid

   #131753

   Purpose

   Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Inserts

   Ad5 E1A gene fragment
   Ad5 Hexon gene fragment
   

   Use

   Standard for qrt-pcr

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUDP293

   Plasmid

   #204228

   Purpose

   Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Inserts

   Eubacterium rectale cas12a (Ercas12a)
   BsaI_GFP drop-out_BsaI
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1 and Saccharomyces c…

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR1055

   Plasmid

   #208741

   Purpose

   Characterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PHrcA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PHrcA

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a Ov-iPGM

   Plasmid

   #180245

   Purpose

   Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   Onchocerca volvulus iPGM-10His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUDE1093

   Plasmid

   #204227

   Purpose

   Episomal yeast plasmid expressing the Ercas12a

   Depositor

   Jean-Marc Daran

   Article

   Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

   Insert

   Eubacterium rectale cas12a (Ercas12a)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   codon optimized for S. cerevisiae

   Promoter

   Saccharomyces cerevisiae PGK1

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM4663

    Plasmid

    #85126

    Purpose

    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

    Depositor

    Susan Golden

    Article

    Chang et al Science. 2015 Jul 17;349(6245):324-8. do

    Insert

    PkaiB-luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM4731

    Plasmid

    #85127

    Purpose

    NSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.

    Depositor

    Susan Golden

    Article

    Chang et al Science. 2015 Jul 17;349(6245):324-8. do

    Insert

    PkaiBC-KaiB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. BAP1-001

    Plasmid

    #68365

    Purpose

    stable expression of BAP1 in mammalian cells

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Parrotta et al J Thorac Oncol. 2017 Apr 4. pii: S1556-0

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

13. BAP1-010

    Plasmid

    #68366

    Purpose

    stable expression of BAP1 delta 221-238 in mammalian cells

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Parrotta et al J Thorac Oncol. 2017 Apr 4. pii: S1556-0

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta 221-238, splice variant found in human meso…

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pQCasTns(Ptr)-entry(BsaI)

    Plasmid

    #190274

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Insert

    PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(BsaI)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSm5-Bsd-P2a-Fkbp-3xF-GGSG

    Plasmid

    #235522

    Purpose

    N-terminal tagging vector for Smarca5

    Depositor

    Brian Hendrich

    Insert

    Smarca5 (Smarca5 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Bsd, FKBP, 3xFLAG

    Expression

    Mutation

    None

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCrl

    Plasmid

    #122443

    Purpose

    Expresses entire mouse reelin ORF in mammalian cells

    Depositor

    Tom Curran

    Article

    D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

    Insert

    reelin (Reln Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS-AMVpl

    Plasmid

    #21738

    Depositor

    Henry Lester

    Article

    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

    Insert

    AMV-pA50
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCrlM

    Plasmid

    #122444

    Purpose

    Expresses myc tagged mouse reelin ORF in mammalian cells

    Depositor

    Tom Curran

    Article

    D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

    Insert

    reelin (Reln Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hulk-ubi-lacZ-to-Green

    Plasmid

    #108641

    Purpose

    ubiquitous promoter followed by a lacZ to GFP lineage tracing line. Cryst:venusGFP

    Depositor

    Nadia Mercader Huber

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Insert

    ubi-lacZ-to-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQCasTns(Ptr)-array 8

    Plasmid

    #175580

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NO

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge