We narrowed to 649 results for: pBR322

1. Bxb1-GT_CAG-mRuby2

   Plasmid

   #229801

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GA_CAG-EGFP

   Plasmid

   #229799

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRL25

   Plasmid

   #70253

   Purpose

   Shuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains

   Depositor

   Peter Wolk

   Article

   Wolk et al J Bacteriol. 1988 Mar;170(3):1239-44.

   Type

   Empty backbone

   Use

   Bacterial shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Bxb1-GA Donor_hEF1a

   Plasmid

   #229779

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

   Plasmid

   #228360

   Purpose

   hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Rfxcas13d grna expression …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105 and tet

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Bxb1-GA_CAG-mStayGold

   Plasmid

   #229802

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mStayGold
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GA Donor_CAG

   Plasmid

   #229780

   Purpose

   Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5155

   Plasmid

   #175391

   Purpose

   Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid

    #199255

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1) nuclease

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

    Insert

    High specificity eSpCas9(1.1) nuclease
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AZ64_pE.DonorCLYBL.TS

    Plasmid

    #199228

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for EGFP reporter
    

    Use

    Gene targeting donor plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1 gene promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. phGR-SB

    Plasmid

    #133409

    Purpose

    overexpression of a human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

    Plasmid

    #139961

    Purpose

    Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. ptrc99a-pVhb-tdTER-fdh

    Plasmid

    #215649

    Purpose

    pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    trans-enoyl-CoA reductase
    formate dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. dSp-VP64-p65-RTA(75-190)

    Plasmid

    #99670

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    VP64-p65-RTA(75-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    May 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. GR A458T

    Plasmid

    #133410

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A458T

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mt SSB

    Plasmid

    #205057

    Purpose

    SSB1 protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    SSB1 (SSB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKS101

    Plasmid

    #24631

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    FAA2 (FAA2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pAL3

    Plasmid

    #114673

    Purpose

    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.

    Depositor

    William Studier

    Article

    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6

    Type

    Empty backbone

    Use

    Tags

    optional Ser-Gly-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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