We narrowed to 910 results for: polya
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Plasmid#175203PurposeXenopus oocyte expression vector containing YBR147W. Contains T7 RNA polymerase binding site, and adds polyA tail to YBR147W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYBR147W
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8153
Plasmid#175204PurposeXenopus oocyte expression vector containing YBR235W. Contains T7 RNA polymerase binding site, and adds polyA tail to YBR235W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYBR235W
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8154
Plasmid#175205PurposeXenopus oocyte expression vector containing YBR287W. Contains T7 RNA polymerase binding site, and adds polyA tail to YBR287W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYBR287W
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8155
Plasmid#175206PurposeXenopus oocyte expression vector containing YCL002C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCL002C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYCL002C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFB8455
Plasmid#175207PurposeXenopus oocyte expression vector containing YCR075C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCR075C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYCR075C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFFLuc-IVT
Plasmid#205147PurposeIVT template of FireFly luciferase stop codon between the ORF and MCS and polyADepositorInsertFireFly luciferase
UseLuciferase; In-vitro transcriptionAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
SV40-MCS-Z1
Plasmid#127639PurposeEmpty backbone plasmid with SV40 promoter and SV40 polyA with Zeocin/Bleocin bacterial resistanceDepositorTypeEmpty backboneExpressionMammalianPromoterSV40 promoterAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRLuc-IVT
Plasmid#205148PurposeIVT template of Renilla luciferase stop codon between the ORF and MCS and polyADepositorInsertRenilla Luciferase
UseLuciferase; In-vitro transcriptionPromoterT7Available SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXA9-MSCV-CD24
Plasmid#20975DepositorUseRetroviralTagsFlagExpressionMammalianMutationFull length cDNA with ~1.1kb 3'UTR with 2 pu…Available SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-Haunt E1-PuroT2AmCherry-4A KI
Plasmid#92164PurposeFor lncRNA 4_pA(3_SV40 polyA and 1 BGH polyA) homologue recombination mediated knockinDepositorInsertHaunt P-Inr2-cDNA KI homology arms
UseOtherAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-EF1a-pA (JDW 1222)
Plasmid#224526PurposeA Gateway compatible 3' entry clone containing rat EF-1a polyADepositorInsertEF-1a polyA
UseGateway cloningAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rich-pT7
Plasmid#108445PurposeThis plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.DepositorTypeEmpty backbonePromoterpT7Available SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCMV3
Plasmid#85711PurposeExpression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequencesDepositorTypeEmpty backboneExpressionMammalianPromoterMIEPmouse CMV MIEPAvailable SinceApril 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hsp68-CreER(TM) (DM#264)
Plasmid#15028DepositorInsertCre-ER(TM)
UseCre/Lox; For making transgenic constructsTagsHsp68 promoter and polyAExpressionMammalianAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xSTOP-lox (JDW 869)
Plasmid#224521PurposeA Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassetteDepositorInsertLoxP-3xStop-LoxP
UseCre/LoxAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterPromoter1.7kb fabp6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only