We narrowed to 2,023 results for: sp6

1. pCS2+methylated-reporter

   Plasmid

   #164491

   Purpose

   Reporter to test methylated mRNA stability, for mRNA injection

   Depositor

   Antonio Giraldez

   Article

   Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

   Insert

   A-less CDS, HA tag, 12x m6A motif 3'UTR
   

   Use

   Production of mrna for zebrafish injection.

   Tags

   HA tag

   Expression

   Mutation

   Promoter

   SP6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT2iU6m5Hoxa13bE4PCh(#765)

   Plasmid

   #184102

   Purpose

   cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   5myc-ZfHoxa13b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes) + in vitro tra…

   Tags

   5xMyc

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi+SP6

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-OXER1-mKate2

   Plasmid

   #226713

   Purpose

   overexpression red fluorescent OXER1 protein in zebrafish larvae

   Depositor

   Philipp Niethammer

   Article

   Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

   Insert

   OXER1 (OXER1 Human)
   

   Use

   Unspecified; Zebrafish expression

   Tags

   mKate2

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-Bmp7

   Plasmid

   #201909

   Purpose

   in vitro transcription of RNA encoding FLAG-tagged Bmp7a

   Depositor

   Mary Mullins

   Article

   Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

   Insert

   bmp7 (bmp7a Zebrafish)
   

   Use

   Tags

   FLAG tag

   Expression

   Mutation

   Promoter

   SP6

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Tol1.zf1

   Plasmid

   #61388

   Purpose

   mRNA synthesis of tol1 transposase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   tol1.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-CaaX(Hras)

   Plasmid

   #108886

   Purpose

   Expression of membrane localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   mCherry-CaaX(Hras)
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MX2_pCSdest

   Plasmid

   #53942

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   MX2 (MX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCS2-Cre.zf1

   Plasmid

   #61391

   Purpose

   mRNA synthesis of Cre recombinase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cre.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. nls-mCherry

   Plasmid

   #108881

   Purpose

   Expression of nuclear localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   nls-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2-Gal4ff.zf1

    Plasmid

    #61392

    Purpose

    mRNA synthesis of Gal4ff codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Gal4ff.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NRIP1_pCSdest

    Plasmid

    #53805

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    NRIP1 (NRIP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

12. PWP2_pCSdest

    Plasmid

    #53946

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PWP2 (PWP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

13. USP25_pCSdest

    Plasmid

    #53817

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    USP25 (USP25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

14. H2B-mCherry

    Plasmid

    #108880

    Purpose

    Expression of human histone2B fused to mCherry (nuclear marker)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    H2B-mCherry
    

    Use

    Can be used for mammalian expression or in vitro …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCiVSP(C363S)-SD64TF

    Plasmid

    #80334

    Purpose

    Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

    Depositor

    Yasushi Okamura

    Article

    Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

    Insert

    Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Changed Cysteine 363 to Serine

    Promoter

    SP6

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-NfsB.zf1

    Plasmid

    #61387

    Purpose

    mRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    nfsB.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    E28G

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2+_NLS-Tir1-HA

    Plasmid

    #117716

    Purpose

    mRNA synthesis of NLS-TIR1-HA for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized TIR1 for murine expression

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. myc-mULK1

    Plasmid

    #31960

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (Ulk1 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

19. myc-hULK2

    Plasmid

    #31966

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK2 (ULK2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pTK095

    Plasmid

    #59560

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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