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pEE388

Plasmid

#149286

Purpose

T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_mFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
DPY-pNCS

Plasmid

#17365

Depositor

Stephen Goff

Article

Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

Insert

MMLV proviral DNA

Mutation

deletion that removes 5 amino acids (DPPPY) from …

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pαH-NiV-F-WT-Foldon

Plasmid

#200391

Purpose

Mammalian cell expression of Nipah virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

NiV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-LayV-F-WT-Foldon

Plasmid

#200392

Purpose

Mammalian cell expression of Langya virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

LayV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Mutation

Ectodomain (AA 1-483)

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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WNV isolate WN NY 2000-crow3356 NS5 RdRp full length

Plasmid

#228662

Purpose

Bacterial expression of the West Nile virus NS5 RdRp protein for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

West nile NS5 RdRp

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRepCap6

Plasmid

#110770

Purpose

pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

Depositor

David Russell

Article

Rutledge et al J Virol. 1998 Jan;72(1):309-19.

Insert

AAV6 cap, AAV6 rep genes

Use

AAV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS5A

Plasmid

#17646

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS5A (POLY Hepatitis C virus)

Tags

Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS3

Plasmid

#17645

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS3 (POLY Hepatitis C virus)

Tags

Flag

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-FLAG-Core-R (pMO29)

Plasmid

#24480

Depositor

Melanie Ott

Article

Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

Insert

Core

Tags

FLAG

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pHBV-S1-Luc

Plasmid

#71416

Purpose

To see S1 promoter activity of HBV for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S1 promoter driving Luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV S1

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHBV-S2-Luc

Plasmid

#71417

Purpose

To see HBV S2 promoter activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV S2 promoter driving luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV S2

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pVSVΔG-eGFP-linker

Plasmid

#231707

Purpose

Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

Depositor

Ron Geller

Article

Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

Insert

VSV antigenome

Use

Vsv expression

Expression

Mammalian

Mutation

ΔG (VSV glycoprotein removed)

Promoter

T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCaRNA1&2

Plasmid

#86087

Purpose

Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

Depositor

Aiming Wang

Article

Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

Insert

RNA1, RNA2

Expression

Plant

Promoter

35S

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTcDNA

Plasmid

#1779

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTgenomic

Plasmid

#1778

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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pHBV-Luc

Plasmid

#71414

Purpose

To see the activity of HBV transcription using Luciferase assay

Depositor

Wang-Shick Ryu

Article

Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

Insert

HBV core promoter, enhancer I, enhancer II and luciferase

Use

Luciferase

Expression

Mammalian

Promoter

HBV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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Tet-inducible- FLAG-Core R (pMO30)

Plasmid

#24479

Depositor

Melanie Ott

Article

Schwer et al J Virol. 2004 Aug . 78(15):7958-68.

Insert

Core R

Tags

FLAG

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pEE393

Plasmid

#149283

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only