We narrowed to 19,059 results for: ire;

1. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

   Plasmid

   #208696

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX304 3xFlag-eRF1 G183/184A_IRES-GFP

   Plasmid

   #198394

   Purpose

   eRF1 (AAQ mutant) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   eRF1 (ETF1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   mutated GGQ motif (G183/184A)

   Promoter

   CMV

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSH-CAG-AtAFB2.F74A-IRES-puro

   Plasmid

   #216245

   Purpose

   Express AtAFB2.F74A mutant without tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CMV-rtTA-IRES-GFP-WPRE-pA

   Plasmid

   #183709

   Purpose

   AAV designed to express rtTA and eGFP from a CMV promoter

   Depositor

   Jonathan Ploski

   Insert

   rtTA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-EF1a-mCherry-MinD-IRES-Puro

   Plasmid

   #213112

   Purpose

   Expression of mCherry-MinD in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

   Plasmid

   #102889

   Purpose

   PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

   Plasmid

   #113886

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

   Plasmid

   #32476

   Purpose

   Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAM Y115F,L141F-5HT3 HC
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50455

    Purpose

    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge